Leptin 抗体
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- 抗原 See all Leptin (LEP) 抗体
- Leptin (LEP)
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适用
- 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Leptin antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 特异性
- Mouse Leptin
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Murine Leptin.
Type of Immunogen: Cell extract - Top Product
- Discover our top product LEP Primary Antibody
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anti-Leptin (LEP) (AA 22-167) antibody
LEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 17-100) antibodyLEP 适用: 人, 小鼠, 大鼠 WB, ELISA, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 8-37) antibodyLEP 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 29-160) antibodyLEP 适用: Cow WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) antibodyLEP 适用: 人, 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 22-167) antibodyLEP 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 7-37), (N-Term) antibodyLEP 适用: 人 WB, IF, FACS, EIA, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Leptin (LEP) (AA 101-154) antibodyLEP 适用: 小鼠, 大鼠 ELISA, FACS, IF (p), IF (cc), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, WB (0.1 - 0.2 μg/mL)
Usage: Immunohistochemistry: This antibody stained 4 % PFA, paraffin-embedded sections of Trichuris muris infected murine tissue. The primary antibody was incubated at 0.20 μg/mL overnight at 4 °C and the secondary antibody was a cyanine-3 conjugated Donkey anti-Rabbit (Jackson ImmunoResearch). Heat induced antigen retrieval with a 100 mM citric acid was used. Information and photo are courtesy of David Artis, University of Pennsylvania. Optimal concentrations and conditions may vary. ELISA: To detect mLeptin by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Murine Leptin (LS-C104759) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant mLeptin. Western Blot: To detect mLeptin by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant mLeptin is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Reconstitute with 50 μL sterile ddH2O.
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- Leptin (LEP)
- 别名
- Leptin (LEP 产品)
- 别名
- ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
- 背景
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Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB - 基因ID
- 3952
- UniProt
- P41159
- 途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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