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Leptin 抗体 (AA 22-167)

LEP 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
产品编号 ABIN7445093
发货至: 中国
  • 抗原 See all Leptin (LEP) 抗体
    Leptin (LEP)
    抗原表位
    • 24
    • 16
    • 15
    • 15
    • 12
    • 11
    • 7
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 22-167
    适用
    • 113
    • 73
    • 71
    • 12
    • 8
    • 7
    • 5
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    宿主
    • 162
    • 23
    • 6
    • 5
    • 2
    克隆类型
    • 173
    • 24
    多克隆
    标记
    • 99
    • 25
    • 16
    • 8
    • 7
    • 6
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    This Leptin antibody is un-conjugated
    应用范围
    • 126
    • 79
    • 63
    • 39
    • 39
    • 38
    • 32
    • 29
    • 24
    • 21
    • 16
    • 11
    • 11
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
    原理
    Polyclonal Antibody to Leptin (LEP)
    特异性
    The antibody is a rabbit polyclonal antibody raised against LEP. It has been selected for its ability to recognize LEP in immunohistochemical staining and western blotting.
    交叉反应
    小鼠, Pig
    纯化方法
    Antigen-specific affinity chromatography followed by Protein A affinity chromatography
    免疫原
    Recombinant Leptin (LEP) corresdonding to Val22~Cys167 with N-terminal His and GST Tag
    亚型
    IgG
  • 应用备注

    Western blotting: 0.5-2 μg/mL

    1:500-2000 Immunohistochemistry: 5-20 μg/mL

    1:50-200 Immunocytochemistry: 5-20 μg/mL

    1:50-200 Optimal working dilutions must be determined by end user.

    说明

    The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

    限制
    仅限研究用
  • 状态
    Liquid
    缓冲液
    0.01M PBS, pH 7.4, containing 0.05 % Proclin-300, 50 % glycerol.
    储存液
    ProClin
    注意事项
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
    有效期
    24 months
  • 抗原
    Leptin (LEP)
    别名
    Leptin (LEP 产品)
    别名
    ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
    背景
    OB, OBS, Obesity Homolog, Obesity Factor, Obese Protein
    途径
    JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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