Leptin 抗体 (N-Term)
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- 抗原 See all Leptin (LEP) 抗体
- Leptin (LEP)
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抗原表位
- N-Term
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适用
- 人, 小鼠, Cow, 马, 犬, 兔, Hamster
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Leptin antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 品牌
- IHC-plus™
- 特异性
- Human LEP / Leptin
- 预测反应
- Percent identity by BLAST analysis: Human, Mouse (100%) Rat (92%) Sheep, Goat (90%) Dog, Bovine (85%).
- 纯化方法
- Immunoaffinity purified
- 免疫原
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Synthetic peptide from N-Terminus of human LEP (P41159, NP_000221). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Orangutan, Gibbon, Mouse, Hamster (100%), Galago, Marmoset, Rat, Opossum (92%), Sheep, Goat (90%), Monkey, Elephant, Zebu, Dog, Cat, Bovine (85%).
Type of Immunogen: Synthetic peptide - 亚型
- IgG
- Top Product
- Discover our top product LEP Primary Antibody
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- 应用备注
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Approved: IHC, IHC-P (5 μg/mL), WB (0.2 - 1 μg/mL)
Usage: Western Blot: Suggested dilution at 1 μg/mL in 5 % skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1: 50,000 - 100,000 as second antibody. ELISA titer in peptide based assay: 1:62500. - 说明
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Target Species of Antibody: Human
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Distilled water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS with 2 % sucrose
- 注意事项
- Avoid repeat freeze-thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Long term: -20°C, the use of 50% glycerol is recommended if storing aliquots in -20°C for long term use (up to 1 year)
Short term (less than 1 week): 4°C. Avoid freeze-thaw cycles.
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- 抗原
- Leptin (LEP)
- 别名
- Leptin (LEP 产品)
- 别名
- ob antibody, obese antibody, LEPD antibody, OB antibody, OBS antibody, leptin antibody, Lep antibody, LEP antibody, lep antibody
- 背景
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Name/Gene ID: LEP
Synonyms: LEP, Leptin, LEPD, Obese protein, Obese, mouse, homolog of, Obesity factor, OBS, OB - 基因ID
- 3952
- NCBI登录号
- NP_000221
- UniProt
- P41159
- 途径
- JAK/STAT Signaling, AMPK Signaling, Hormone Transport, Peptide Hormone Metabolism, Hormone Activity, Negative Regulation of Hormone Secretion, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Monocarboxylic Acid Catabolic Process
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