CCL2 抗体
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- 抗原 See all CCL2 抗体
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
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适用
- 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This CCL2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Neutralization (Neut)
- 特异性
- Rat MCP-1
- 纯化方法
- Immunoaffinity purified
- 免疫原
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E.coli derived recombinant Rat MCP-1.
Type of Immunogen: Cell extract - Top Product
- Discover our top product CCL2 Primary Antibody
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- 应用备注
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Approved: ELISA (0.5 - 2 μg/mL), Neut (4 - 6 μg/mL), WB
Usage: Immunohistochemistry: This antibody stained colchicine injected rat brain (including the caudate putamen) tissue. Frozen samples fixed in 4 % PFA. The primary antibody was incubated at 1 μg/mL overnight at 4 °C. This was followed by a peroxidase conjugated secondary antibody and then a fluorescein Tyramide Signal Amplification (TSA) reagent. Optimal concentrations and conditions may vary. Information and photo are courtesy of the Tissue Profiling group, SciLifeLab Stockholm. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Rat MCP-1 (MCAF) (100 ng/mL), a concentration of 4-6 μg/mL of this antibody is required. ELISA: To detect Rat MCP-1(MCAF) by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5-2 μg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Rat MCP-1(MCAF) (LS-C104762) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Rat MCP-1(MCAF). Western Blot: To detect Rat MCP-1 (MCAF) by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant Rat MCP-1 (MCAF) is 1.5-3 ng/lane, under either reducing or non-reducing conditions. - 说明
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Target Species of Antibody: Rat
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Sterile water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized from PBS, pH 7.2
- 注意事项
- Avoid freeze/thaw cycles.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Store Lyophilized at room temperature up to 1 month
Reconstituted for up to 2 weeks at 2-8°C. Aliquot and freeze at -20°C for long term storage. Avoid freeze/thaw cycles.
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- 抗原
- CCL2 (Chemokine (C-C Motif) Ligand 2 (CCL2))
- 别名
- CCL2 / MCP-1 (CCL2 产品)
- 别名
- GDCF-2 antibody, HC11 antibody, HSMCR30 antibody, MCAF antibody, MCP-1 antibody, MCP1 antibody, SCYA2 antibody, SMC-CF antibody, AI323594 antibody, JE antibody, Scya2 antibody, Sigje antibody, MCP-1A antibody, MCP1A antibody, C-C motif chemokine ligand 2 antibody, chemokine (C-C motif) ligand 2 antibody, C-C motif chemokine 2 antibody, CCL2 antibody, Ccl2 antibody, LOC101120093 antibody
- 背景
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Name/Gene ID: CCL2
Subfamily: Intercrine beta
Family: Intercrine
Synonyms: CCL2, C-C motif chemokine 2, Chemokine (C-C motif) ligand 2, GDCF-2, HC11, HSMCR30, MCP1, SCYA2, Small-inducible cytokine A2, Monocyte secretory protein JE, MCAF, MCP-1, Monocyte chemotactic protein 1, SMC-CF - 基因ID
- 6347
- UniProt
- P13500
- 途径
- Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Immune Effector Process, ER-Nucleus Signaling, Unfolded Protein Response, The Global Phosphorylation Landscape of SARS-CoV-2 Infection
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