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小鼠 anti-人 IgE Antibody (Biotin)
ELISA, WB, FACS, Func
Monoclonal
Le27-NBS01
IgG1 kappa
Biotin
产品细节
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抗原
See all IgE products
IgE
适用
All reactivities for IgE Secondaries
人
宿主
All hosts for IgE Secondaries
小鼠
克隆类型
All clonalities for IgE Secondaries
单克隆
标记
All conjugates for IgE Secondaries
Biotin
应用范围
All applications for IgE Secondaries
ELISA, Western Blotting (WB), Flow Cytometry (FACS), Functional Studies (Func)
特异性
Human IgE, Fce4, no cross-ractivity with other human or murine Ab isotypes
灵敏度
Reactivity > 95 %, compared to calibrator (Internal Standard mAb clone Le27), tested by ELISA binding to immobilized human Standard IgE (clone SUS-11)
产品特性
IGHE, chromosome 14q32.33
纯化方法
Affinity - purified from cell culture supernatant by Immunoaffinity Chromatography on CH-Sepharose 4B - rat monoclonal anti- mouse Ck
纯度
> 95 %, tested by analytical Gel Permeation Chromatography
过滤
Sterile filtered
免疫原
purified human myeloma IgE
克隆位点
Le27-NBS01
亚型
IgG1 kappa
Alternatives
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使用细节
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应用备注
For functional cellular assays (mast cell / basophil activation), in particular when used at dilutions < 1:100
限制
仅限研究用
贮存及处理
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浓度
1 mg/mL
缓冲液
PBS, pH=7.2, sterile filtered, contains no NaN3
储存液
Azide free
注意事项
Aliquot to Avoid repeated freezing and thawing.
储存条件
4 °C
目标详细情况
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抗原
IgE
Abstract
IgE 产品
背景
The antibody recognizes an epitope within the Ce4 domain of human IgE. It does not block the binding of IgE to its high affinity receptor on mast cells and basophils and is therefore suitable to detect receptor bound IgE. It can also be used as cross-linking agent for allergen-independent triggering of mast cells and basophils.
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IgE
小鼠 anti-人 IgE Antibody (Biotin) (ABIN457499)