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小鼠 anti-人 IgE Antibody (PE)

FACS Monoclonal BE5 IgG1 PE
产品编号 ABIN192257
发货至: 中国
  • 抗原 See all IgE products
    IgE
    适用
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    宿主
    • 58
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    小鼠
    克隆类型
    • 66
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    单克隆
    标记
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    PE
    应用范围
    • 55
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    Flow Cytometry (FACS)
    特异性
    The antibody BE5 reacts with human IgE, it recognizes an epitope different from the ones recognized by 4G7 and 4H10 antibodies to IgE.
    交叉反应 (详细)
    Human
    纯化方法
    Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
    免疫原
    Purified human IgE.
    克隆位点
    BE5
    亚型
    IgG1
  • 应用备注
    Flow cytometry: Recommended dilution: 2-5 μg/mL.
    说明

    The purified antibody is conjugated with R-Phycoerythrin (PE) under optimum conditions. The conjugate is purified by size-exclusion chromatography.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.1 mg/mL
    缓冲液
    Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Do not freeze.
    Avoid prolonged exposure to light.
    储存条件
    4 °C
    储存方法
    Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
  • Kaulfürst-Soboll, Mertens, Brehler, von Schaewen: "Reduction of cross-reactive carbohydrate determinants in plant foodstuff: elucidation of clinical relevance and implications for allergy diagnosis." in: PLoS ONE, Vol. 6, Issue 3, pp. e17800, (2011) (PubMed).

  • 抗原
    IgE
    Abstract
    IgE 产品
    背景
    Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E
    分子量
    180 kDa
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