IREB2 抗体 (Internal Region)
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- 抗原 See all IREB2 抗体
- IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))
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抗原表位
- Internal Region
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适用
- 人, 大鼠, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IREB2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- c-SIHYEGSEYK LSHGS
- 特异性
- This antibody recognizes Iron Regulatory Protein 2 (IREB2/IRP2).
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence from the internal region of IREB2 (IRP2) according to NP_004127.1. Genename: IREB2
- Top Product
- Discover our top product IREB2 Primary Antibody
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anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 39-281) antibody
IREB2 适用: 人 WB, ELISA, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 107-216) antibodyIREB2 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 1-343) antibodyIREB2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (Internal Region) antibodyVerified IREB2 适用: 人 WB, ELISA, IHC 宿主: 山羊 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 100-200) antibodyIREB2 适用: 人, 大鼠, 小鼠, 灵长类 WB 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 1-250) antibodyIREB2 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 1-343) antibodyIREB2 适用: 大鼠 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 201-230) antibodyIREB2 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB30212 unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (Middle Region) antibodyIREB2 适用: 人, 大鼠, 小鼠, Pig, 犬, 马, 豚鼠, 兔, 斑马鱼 WB 宿主: 兔 Polyclonal unconjugated
anti-Iron-Responsive Element Binding Protein 2 (IREB2) (AA 208-238), (Middle Region) antibodyIREB2 适用: 人 WB, FACS, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Peptide ELISA: IREB2 Antibody Detection limit dilution 1/32000. Western blot: 1-3 μg/mL. Approx 110 kDa band observed in Human Liver lysates (calculatedMW of 100 kDa according to NP_004127.1). An additional band of unknown identity wasalso consistently observed at 60 kDa. This band was successfully blocked by incubationwith the immunizing peptide.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- 抗原
- IREB2 (Iron-Responsive Element Binding Protein 2 (IREB2))
- 别名
- IREB2 (IREB2 产品)
- 别名
- IREB2 antibody, im:7153062 antibody, DKFZp468N047 antibody, D9Ertd85e antibody, Irp2 antibody, ACO3 antibody, IRP2 antibody, IRP2AD antibody, IREBP2 antibody, iron responsive element binding protein 2 antibody, iron-responsive element binding protein 2 antibody, iron responsive element binding protein 2 L homeolog antibody, IREB2 antibody, ireb2 antibody, Ireb2 antibody, ireb2.L antibody
- 背景
- Iron regulatory protein 2 (IREB2 or IRP2) is a central regulator of cellular iron homeostasis due to its regulation of specific mRNAs encoding proteins of iron uptake and storage. In mammalian cells, regulation of the expression of proteins involved in iron metabolism is achieved through interactions of iron-sensing proteins known as iron regulatory proteins (IRPs), with transcripts that contain RNA stem-loop structures referred to as iron responsive elements (IREs). Two distinct but highly homologous proteins, IRP1 and IRP2, bind IREs with high affinity when cells are depleted of iron, inhibiting translation of some transcripts, such as ferritin, or turnover of others, such as the transferrin receptor (TFRC). IRPs sense cytosolic iron levels and modify expression of proteins involved in iron uptake, export and sequestration according to the needs of individual cells. Regulating the amount of iron sequestered by ferritin and taken up by transferrin receptor ensures that cells acquire sufficient iron for their needs, while preventing iron toxicity.Synonyms: IRE-BP2, IRP2, Iron regulatory protein 2, Iron-responsive element-binding protein 2
- 基因ID
- 3658
- NCBI登录号
- NP_004127
- UniProt
- P48200
- 途径
- Transition Metal Ion Homeostasis
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