(1 reference)-
- 抗原 See all SPRY1 抗体
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SPRY1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This antibody detects Sprouty at C-term.
- 交叉反应 （详细）
- Species reactivity (tested):Human and Mouse.
- 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.
- Top Product
- Discover our top product SPRY1 Primary Antibody
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anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-110) antibody
SPRY1 适用: 人 WB, ELISA, IHC (p) 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 112-303) antibodySPRY1 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (C-Term) antibodyVerified SPRY1 适用: 人, 小鼠 WB, ELISA, IHC, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-178) antibodySPRY1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) antibodySPRY1 适用: 人 WB, IHC (p), EIA 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-110) antibodySPRY1 适用: 人 WB, ELISA 宿主: 小鼠 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-319) antibodySPRY1 适用: 人 WB, IP 宿主: 兔 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-111) antibodySPRY1 适用: 人 WB, ELISA, IHC, IHC (p) 宿主: 小鼠 Monoclonal 3H4 unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 118-309) antibodySPRY1 适用: 人 WB, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1) (AA 1-319) antibodySPRY1 适用: 人 WB 宿主: 小鼠 Polyclonal unconjugated
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- 应用备注
- Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL (Successfully used in ERMS cells, PMID: 20068162). Immunohistochemistry: 1-3 μg/mL (Successfully used by anonymous customers in paraffinembedded Mouse Brain, showing nuclear staining).
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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: "Atypical role of sprouty in p21 dependent inhibition of cell proliferation in colorectal cancer." in: Molecular carcinogenesis, Vol. 55, Issue 9, pp. 1355-68, (2016) (PubMed).
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: "Atypical role of sprouty in p21 dependent inhibition of cell proliferation in colorectal cancer." in: Molecular carcinogenesis, Vol. 55, Issue 9, pp. 1355-68, (2016) (PubMed).
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- 抗原
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- 别名
- Sprouty Homolog 1 / SPRY1 (SPRY1 产品)
- 别名
- hSPRY1 antibody, SPRY1 antibody, LOC100223875 antibody, hspry1 antibody, xspry1 antibody, sprouty antibody, sprouty1 antibody, sprouty-1 antibody, Xtsprouty1 antibody, spry1a antibody, spry1b antibody, sprouty RTK signaling antagonist 1 antibody, sprouty RTK signaling antagonist 1 L homeolog antibody, sprouty RTK signaling antagonist 1 S homeolog antibody, sprouty homolog 1 (Drosophila) antibody, SPRY1 antibody, Spry1 antibody, spry1 antibody, spry1.L antibody, spry1.S antibody
- 背景
- Members of the Sprouty family (Sprouty 1-4) are inducible negative regulators of growth factors that act through tyrosine kinase receptors. Mammalian Sprouty homologs share a well conserved cysteine-rich C-terminal domain with their Drosophilacounterpart. Both Sprouty 1 and 2 are anchored to membranes by palmitoylation, associate with caveolin-1 in perinuclear and vesicular structures and are phosphorylated on Serine residues. Upon stimulation, a subset is recruited to the leading edge of the plasma membrane. Sprouty 2 can associate with c-Cbl, a downregulator of RTK signaling, and inhibits the activities of several growth factors. Sprouty 2 also functions as a negative regulator of embryonic lung morphogenesis and growth. The wellconserved C-terminus of Sprouty contains two domains which are necessary for Sprouty 2 colocalization with microtubules and translocation to membrane ruffles. In addition, the C-terminus is required for the inhibition of cell migration and proliferation. In conclusion, members of Sprouty inhibit FGF and VEGF-mediated cell proliferation, suggesting that they may regulate angiogenesis in normal and disease processes.Synonyms: Sprouty 1, Spry-1
- 基因ID
- 10252
- NCBI登录号
- NP_005832
- UniProt
- O43609
- 途径
- EGFR Signaling Pathway, EGFR Downregulation
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