SPRY1 抗体 (C-Term)
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- 抗原 See all SPRY1 抗体
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
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抗原表位
- C-Term
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This SPRY1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Flow Cytometry (FACS)
- 原理
- Sprouty
- 序列
- CPSRGQGKPS
- 特异性
- NP_005832.1 and NP_955359.1 represent varients of the same protein.
- 交叉反应
- 人
- 纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- 质量等级
- Verified
- 免疫原
- Peptide with sequence CPSRGQGKPS, from the C Terminus of the protein sequence according to NP_005832.1, NP_955359.1.
- 亚型
- IgG
- Top Product
- Discover our top product SPRY1 Primary Antibody
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- 应用备注
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Immunohistochemistry: This product has been successfully used by customers in paraffin embedded Mouse Brain, showing nuclear staining. Recommended concentration: 1-3 μg/mL.
Western Blot: Approx 37 kDa band observed in Human Kidney lysates (calculated MW of 35.1 kDa according to NP_005832.1 ). Recommended concentration: 0.3-1 μg/mL. Primary incubation 1 hour at room temperature. This.product has been successfully used in ERMS
Peptide ELISA: antibody detection limit dilution 1:16000.
- 说明
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Immunofluorescence: Strong expression of the protein seen in the cytoplasm and Golgi apparatus of HeLa and HepG2 cells. Recommended concentration: 10μg/ml.
Flow Cytometry: Flow cytometric analysis of HEK293 cells. Rec
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Minimize freezing and thawing.
- 储存条件
- -20 °C
- 储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- 抗原
- SPRY1 (Sprouty Homolog 1, Antagonist of FGF Signaling (SPRY1))
- 别名
- SPRY1 (SPRY1 产品)
- 别名
- hSPRY1 antibody, SPRY1 antibody, LOC100223875 antibody, hspry1 antibody, xspry1 antibody, sprouty antibody, sprouty1 antibody, sprouty-1 antibody, Xtsprouty1 antibody, spry1a antibody, spry1b antibody, sprouty RTK signaling antagonist 1 antibody, sprouty RTK signaling antagonist 1 L homeolog antibody, sprouty RTK signaling antagonist 1 S homeolog antibody, sprouty homolog 1 (Drosophila) antibody, SPRY1 antibody, Spry1 antibody, spry1 antibody, spry1.L antibody, spry1.S antibody
- 背景
- SPRY1, hSPRY1, sprouty homolog 1, antagonist of FGF signaling (Drosophila), sprouty (Drosophila) homolog 1 (antagonist of FGF signaling), sprouty, Drosophila, homolog of, 1 (antagonist of FGF signaling)
- 基因ID
- 10252
- NCBI登录号
- NP_005832, NP_955359
- 途径
- EGFR Signaling Pathway, EGFR Downregulation
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