ROCK2 抗体 (pTyr256)
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- 抗原 See all ROCK2 抗体
- ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
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抗原表位
- pTyr256
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适用
- 人, 小鼠, 大鼠, Cow, 犬, 小鸡, Drosophila melanogaster, 非洲爪蟾
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ROCK2 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This phospho-specific polyclonal antibody reacts with Human ROCK-2 pTyr256 and shows minimal reactivity by ELISA against the non-phosphorylated form of the immunizing peptide. Expect a band approximately 164 kDa in size corresponding to phosphorylated ROCK-2
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Chicken, Canine, Bovine, Xenopus, Drosophila.
Species reactivity (tested):Human. - 纯化方法
- Affinity Chromatography.
- 免疫原
- Synthetic peptide corresponding to residues surrounding Y256 of human ROCK-2
- 亚型
- IgG
- Top Product
- Discover our top product ROCK2 Primary Antibody
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anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 904-1140) antibody
ROCK2 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 1279-1388) antibodyROCK2 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 1E12 unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) antibodyKO Validated ROCK2 适用: 人 WB, IP, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 1001-1300) antibodyROCK2 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 1109-1388) antibodyROCK2 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 652-923) antibodyROCK2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 1279-1388) antibodyROCK2 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 2A4 unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (N-Term) antibodyROCK2 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) antibodyROCK2 适用: 人 WB, ELISA, IF 宿主: 兔 Monoclonal 10E1 unconjugated Recombinant Antibody
anti-rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2) (AA 250-350) antibodyROCK2 适用: 人, 小鼠, 大鼠, Cow, 犬, 小鸡 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1/2,000-1/10,000. Western Blot: 1/200-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.1 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide, None
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-28 °C for longer.
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- 抗原
- ROCK2 (rho-Associated, Coiled-Coil Containing Protein Kinase 2 (ROCK2))
- 别名
- ROCK2 (ROCK2 产品)
- 别名
- rocka-a antibody, xROK antibody, rock2 antibody, rok2 antibody, ROCK2 antibody, B230113H15Rik antibody, ROKalpha antibody, Rho-kinase antibody, Rock-II antibody, Rock2m antibody, mKIAA0619 antibody, ROCK-II antibody, ROK antibody, Rho-associated, coiled-coil containing protein kinase 2 L homeolog antibody, rho-associated, coiled-coil containing protein kinase 2a antibody, Rho associated coiled-coil containing protein kinase 2 antibody, Rho-associated, coiled-coil containing protein kinase 2 antibody, Rho-associated coiled-coil containing protein kinase 2 antibody, rock2.L antibody, rock2a antibody, ROCK2 antibody, Rock2 antibody
- 背景
- Progression of tumors to invasive and metastatic forms requires that tumor cells undergo dramatic morphological changes, a process regulated by Rho GTPases. Increased levels of RhoA or Rh °C, as well as the Rho effector proteins ROCK-1 or ROCK-2, were observed in various human carcinomas. In vivo studies have also shown that ROCK inhibition reduced the invasiveness of several tumor cell lines. It is suggested that Rho and ROCK signaling contribute to the morphological changes and metastatic behavior of some tumor cells. Thus, elevated Rho and ROCK expression is associated with tumorigenesis, particularly during the progression to invasive and metastatic phenotypes. Phosphorylation of certain residues in ROCK-2 may be critical for its kinase activity.Synonyms: KIAA0619, ROCK-2, Rho kinase 2, Rho-associated, Rho-associated protein kinase 2, coiled-coil-containing protein kinase 2, p164ROCK-2
- 基因ID
- 9475
- NCBI登录号
- NP_004841
- UniProt
- O75116
- 途径
- Microtubule Dynamics, WNT signaling, Tube Formation
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