SPRY4 抗体 (AA 306-322)
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- 抗原 See all SPRY4 抗体
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
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抗原表位
- AA 306-322
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适用
- 人, 小鼠, 大鼠, Cow, 犬, Chimpanzee
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This SPRY4 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 特异性
- This affinity purified antibody is directed against Sprouty-4 protein.
- 交叉反应 （详细）
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Species reactivity (expected):Chimpanzee (100 %).Bovine (94 %).Mouse (88 %).Rat and dog (82 %).
Species reactivity (tested):Human. - 纯化方法
- Immunoaffinity chromatography
- 免疫原
- Synthetic peptide corresponding to amino acids 306-322 of Human Sprouty-4 protein
- 亚型
- IgG
- Top Product
- Discover our top product SPRY4 Primary Antibody
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anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) antibody
SPRY4 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Internal Region) antibodySPRY4 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 306-322) antibodySPRY4 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 26-56) antibodySPRY4 适用: 人 WB, FACS, IHC (p) 宿主: 兔 Polyclonal RB15585 unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 1-299) antibodySPRY4 适用: 人 ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (C-Term) antibodySPRY4 适用: 人, 大鼠, 豚鼠, 马, 兔, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (C-Term) antibodySPRY4 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (AA 30-210) antibodySPRY4 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Sprouty RTK Signaling Antagonist 4 (SPRY4) (Middle Region) antibodySPRY4 适用: 人, 小鼠, 大鼠, Cow, 豚鼠, 马, 兔 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 3,000 - 1: 12,000. Western blot: 1: 100 - 1: 1,000 (Expect a band (maybe a doublet) approx. 35 kDa in sizecorresponding to Sprouty-4 in the appropriate cell lysate or extract). Immunohistochemistry on paraffin sections: 1 μg/mL to 10 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 0.93 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % (w/v) Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
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- 抗原
- SPRY4 (Sprouty RTK Signaling Antagonist 4 (SPRY4))
- 别名
- Sprouty Homolog 4 / SPRY4 (SPRY4 产品)
- 别名
- SPRY4 antibody, LOC100218545 antibody, HH17 antibody, A030006O18Rik antibody, sprouty4 antibody, sprouty RTK signaling antagonist 4 antibody, sprouty (Drosophila) homolog 4 antibody, sprouty homolog 4 (Drosophila) antibody, Spry4 antibody, SPRY4 antibody, spry4 antibody
- 背景
- Sprouty-4 (also known as SPRY4) is an inhibitor of the insulin receptor and EGFR-transduced mitogen-activated protein kinase (MAPK) signaling pathway downstream of FGF and EGF receptor tyrosine kinase activation. It is positioned upstream of RAS activation and impairs the formation of active GTP-RAS. SPRY4 is widely expressed, with different isoforms. The protein consists of 322 amino acids, with a cysteine-rich region, Src homology 3 binding regions, proline-rich regions, and a PEST sequence. It is expressed predominantly in the cytoplasm. Northern results show bands across most tissues, with strongest expression in heart, brain, placenta, lung, and intestine.Synonyms: Sprouty 4, Spry-4
- 基因ID
- 81848
- NCBI登录号
- NP_001120968
- UniProt
- Q9C004
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