Species reactivity (expected):Mouse, Rat. Species reactivity (tested):Human.
纯化方法
Affinity chromatography on Protein A
免疫原
This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to amino acids 1-836 of human AGAP2 protein.
AGAP2
适用: 人
WB, ELISA, IF (cc), IF (p), IHC (p), IHC (fro)
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA: 1/40000 - 1/200000. Western Blot: 1/5000 - 1/20000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 50 μL deionized water or equivalent.
浓度
2.0 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Prior to reconstitution store at 2-8 °C. Following reconstitution store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
抗原
AGAP2
(ArfGAP with GTPase Domain, Ankyrin Repeat and PH Domain 2 (AGAP2))
AGAP2 (also known as centauringamma1, ARF-GAP with GTP-binding protein-like, ankyrin repeat and pleckstrin homology domains 2, Phosphatidylinositol-3-kinase enhancer, PIKE, GTP-binding and GTPase activating protein 2 and GGAP2) is a GTPase activating protein that inactivates Arf. The expression of AGAP2 is amplified in human glioblastoma cells. AGAP2 protein binds to and activates the protein kinase Akt and prevents apoptosis of cancer cells. AGAP2 also increases motility and invasive activity of cancer cells. AGAP2 may serve as a diagnostic and therapeutic target of human cancer.Synonyms: Arf-GAP GTPase ANK repeat and PH domain-containing protein 2, Centaurin-gamma-1, GGAP2, GTP-binding and GTPase-activating protein 2, KIAA0167, PIKE, Phosphatidylinositol-3-kinase enhancer