GRP94 抗体 (Internal Region)
-
- 抗原 See all GRP94 (HSP90B1) 抗体
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
-
抗原表位
- Internal Region
-
适用
- 人, 小鼠, 大鼠, Cow, 犬, Pig, 斑马鱼
-
宿主
- 山羊
-
克隆类型
- 多克隆
-
标记
- This GRP94 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- C-KEGVKFDESE KTKE
- 特异性
- This antibody recognizes GPR94/TRA1 (Internal).
- 交叉反应 （详细）
-
Species reactivity (expected):Canine (Dog), Pig, Cow and Zebrafish.
Species reactivity (tested):Human, Mouse, Rat. - 纯化方法
- Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.
- 免疫原
- Peptide with sequence from the internal region of the protein sequence according to NP_003290.1. Genename: HSP90B1
- Top Product
- Discover our top product HSP90B1 Primary Antibody
-
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibody
HSP90B1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibodyHSP90B1 适用: 人, 小鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibodyVerified HSP90B1 适用: 人, 小鼠 WB, IHC, IF, FACS, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 WB, IHC, IF, FACS, StM 宿主: 大鼠 Monoclonal 9G10-F8-2 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 IHC, FACS, StM 宿主: 兔 Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人 WB, IHC, IF, ELISA, IP 宿主: 小鼠 Monoclonal H9010 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2H3 unconjugated
-
- 应用备注
-
Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.01-0.03 μg/mL. Approx 100 kDa band observed in lysates of cell lineNIH/3T3 (calculated MW of 92.0 kDa according to NP_003290.1). Immunohistochemistry: 3-5 μg/mL. In paraffin embedded Human Liver shows staining insome hepatocyte nuclei and weak cytoplasm staining of hepatocytes.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
- 别名
- Endoplasmin / HSP90B1 / TRA1 (HSP90B1 产品)
- 别名
- ecgp antibody, gp96 antibody, tra1 antibody, grp94 antibody, DDBDRAFT_0206450 antibody, DDBDRAFT_0215015 antibody, DDB_0206450 antibody, DDB_0215015 antibody, TRA1 antibody, HSP90B1 antibody, ERp99 antibody, GRP94 antibody, TA-3 antibody, Targ2 antibody, Tra-1 antibody, Tra1 antibody, endoplasmin antibody, GP96 antibody, fb61d09 antibody, tra-1 antibody, wu:fb61d09 antibody, wu:fq25g01 antibody, Grp94 antibody, ECGP antibody, Gp96 antibody, ppk98 antibody, hsp108 antibody, heat shock protein 90kDa beta (Grp94), member 1 antibody, heat shock protein Hsp90 family protein antibody, 94 kD glucose-regulated protein antibody, heat shock protein 90 beta family member 1 antibody, heat shock protein 90, beta (Grp94), member 1 antibody, heat shock protein 90, beta (grp94), member 1 antibody, heat shock protein 90kDa beta family member 1 L homeolog antibody, hsp90b1 antibody, grp94 antibody, HSP90B1 antibody, Hsp90b1 antibody, hsp90b1.L antibody
- 背景
- Glucose regulated protein 94 (GRP 94) is a resident protein of the endoplasmic reticulum (ER) and is induced by the accumulation of unfolded proteins suggesting that it might associate transiently with a variety of newly synthesized secretory and membrane proteins or permanently with mutant or defective proteins. The highly conserved sequence Lys-Asp-Glu-Leu (KDEL) is present at the C terminus of GRP 94 and other resident ER proteins including GRP 78 and protein disulfide isomerase (PDI). The presence of carboxy terminal KDEL appears to be necessary for retention and appears to be sufficient to reduce the secretion of proteins from the ER. This retention is reported to be mediated by a KDEL receptor. GRP 94 is also a low affinity, high capacity calcium binding protein, though it's role, if any, in calcium regulation is not understood.Synonyms: 94 kDa glucose-regulated protein, GRP94, Heat shock protein 90 kDa beta member 1, Tumor rejection antigen 1
- 基因ID
- 7184
- NCBI登录号
- NP_003290
- UniProt
- P14625
- 途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
-
