GRP94 抗体
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北京 101111
Quick Overview for GRP94 抗体 (ABIN6940802)
抗原
See all GRP94 (HSP90B1) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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原理
- GRP94 / HSP90B1 (Endoplasmic Reticulum Marker) Antibody
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特异性
- Recognizes a protein of 94 kDa, which is identified as the glucose-regulated protein 94 (grp94) and also tumor rejection antigen (gp96). Grp94 shows a high degree of sequence homology with the heat shock protein 90 (hsp90). This MAb is highly specific to grp94 and shows minimal cross-reaction with other members of the HSP90 family. Grp's are a class of proteins unresponsive to heat shock and are induced by glucose deprivation. Grp94 has been briefly studied as a prognostic factor in breast cancer.
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纯化方法
- 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.
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免疫原
- Purified glucose regulated protein 94 (grp94) from chicken oviducts
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亚型
- IgG2a, kappa
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anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Center) antibody
HSP90B1 适用: 人, 小鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IF, IP, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 550-650) antibodyHSP90B1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (Internal Region) antibodyVerified HSP90B1 适用: 人, 小鼠 WB, IHC, FACS, IF, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 43-221) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, FACS, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 676-803) antibodyHSP90B1 适用: 人 WB, IF, IHC (p), EIA 宿主: 小鼠 Monoclonal 2H3 unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (C-Term) antibodyHSP90B1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ELISA, ICC 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibodyHSP90B1 适用: 人, 小鼠, 大鼠, Cow, 小鸡, Hamster, 犬, 兔, Pig, 豚鼠, 猴, 绵羊, 马, 非洲爪蟾 WB, IHC, FACS 宿主: 兔 Monoclonal HSP90B1-3168R unconjugated Recombinant Antibody
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) (AA 687-700), (Middle Region) antibodyHSP90B1 适用: 人, 大鼠 WB, IHC, ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1) antibody (Atto 488)HSP90B1 适用: 小鸡 WB, FACS, IF, IP, ICC 宿主: 大鼠 Monoclonal 9G10 Atto 488
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应用备注
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Positive Control: HepG2, U20S cells. About 50 % of breast tumors express grp94 protein.
Known Application: Flow Cytometry (0.5-1 μg/million cells), Immunofluorescence (1-2 μg/mL), Western Blot (0.5-1.0 μg/mL), Immunohistochemistry (Formalin-fixed) (0.5-1.0 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
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限制
- 仅限研究用
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浓度
- 200 μg/mL
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缓冲液
- Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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储存条件
- 4 °C
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储存方法
- Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
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有效期
- 24 months
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- GRP94 (HSP90B1) (Heat Shock Protein 90kDa beta (Grp94), Member 1 (HSP90B1))
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别名
- HSP90B1
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背景
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Glucose Regulated Protein 94, 94 kDa glucose-regulated protein, Endoplasmin, Endothelial cell (HBMEC) glycoprotein (ECGP), Heat shock protein 90 kDa beta member 1 (HSP90B1), Stress inducible tumor rejection antigen GP96, TRA1, Tumor rejection antigen-1 (gp96)
HePG2, U20S cells. About 50 % of breast tumors express grp94 protein.
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分子量
- 94kDa
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基因ID
- 7184, 192374
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UniProt
- P14625
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途径
- Thyroid Hormone Synthesis, Activation of Innate immune Response, ER-Nucleus Signaling, Toll-Like Receptors Cascades
抗原
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