IFT74 抗体 (C-Term)
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- 抗原 See all IFT74 抗体
- IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))
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抗原表位
- C-Term
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适用
- 人, 大鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This IFT74 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- 序列
- C-KTIVDALHST SGN
- 特异性
- This antibody recognizes IFT74/CCDC2 at C-term.
- 交叉反应 （详细）
- Species reactivity (tested):Rat and Human.
- 纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
- 免疫原
- Peptide from the C Terminus of the protein sequence according to NP_001092692.1, NP_001092693.1, NP_079379.2, AAK77221.1. Genename: IFT74
- Top Product
- Discover our top product IFT74 Primary Antibody
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anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (C-Term) antibody
Verified IFT74 适用: 人 ELISA, WB 宿主: 山羊 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) antibodyVerified IFT74 适用: 人 ELISA, IF, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) antibodyIFT74 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (AA 1-100) antibodyIFT74 适用: 人 ELISA, WB 宿主: 小鼠 Monoclonal 2B6 unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (C-Term) antibodyIFT74 适用: 人 WB, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (AA 51-150) antibodyIFT74 适用: 人 ELISA, IF (cc), IF (p), IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (AA 375-600) antibodyIFT74 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) antibodyIFT74 适用: 人 ELISA, WB 宿主: 兔 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) (AA 1-100) antibodyIFT74 适用: 人 ELISA, WB 宿主: 小鼠 Polyclonal unconjugated
anti-Intraflagellar Transport Protein 74 Homolog (IFT74) antibodyIFT74 适用: 人, 大鼠, 小鼠 WB 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Peptide ELISA: Limit dilution 1: 16000. Western blot: 0.3-1 μg/mL. Approx 70-75 kDa band observed in lysates of cell line HEK293(calculated MW of 69.5 kDa according to AAK77221.1, but 51.5 kDa according toNP_079379.1). The observed molecular weight corresponds to earlier findings in literaturewith different antibodies (Momeni et al, BMC Neurol.2006 Dec 13,6: 44. , PMID: 17166276). Immunohistochemistry: 5-10 μg/mL. In paraffin embedded Human Liver shows vesicular staining in cytoplasm of hepatocytes. Immunofluorescence: Successfully used at 2.5 mg/mL to stain rat cortical neurons (see
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH 7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- IFT74 (Intraflagellar Transport Protein 74 Homolog (IFT74))
- 别名
- IFT74 (IFT74 产品)
- 别名
- CCDC2 antibody, DKFZp469F1522 antibody, 1700029H06Rik antibody, Ccdc2 antibody, Cmg1 antibody, b2b796Clo antibody, CMG-1 antibody, CMG1 antibody, intraflagellar transport protein 74/72 antibody, intraflagellar transport 74 antibody, intraflagellar transport protein 74 homolog antibody, IFT74 antibody, ift74 antibody, LOC100632007 antibody, LOC100649180 antibody, Ift74 antibody
- 背景
- Capillary morphogenesis protein 1 (CMG1) is a 600 amino acid protein and has a calculated molecular mass of 65 kD. CMG1 contains a coiled-coil domain (which shares 24 % identity with myosin heavy chain sequences from various species) and several putative phosphorylation sites. Bell et al. (2001) determined that CMG1 was 1 of several transcripts upregulated by umbilical vein endothelial cells during capillary morphogenesis in 3-dimensional collagen matrices. RT-PCR detected highest expression in adult and fetal kidney and lower expression in adult heart, placenta, lung, liver, and pancreas, and in fetal heart, lung, and liver. Little to no expression was detected in adult brain and skeletal muscle or in fetal brain, thymus, and spleen. Transfection into epithelial tumor cells revealed targeting of fluorescent-tagged CMG1 to an intracellular vesicular compartment.Synonyms: CCDC2, CMG-1, CMG1, Capillary morphogenesis protein 1, Coiled-coil domain-containing protein 2, Intraflagellar transport protein 74 homolog
- 基因ID
- 80173
- NCBI登录号
- NP_001092692
- UniProt
- Q96LB3
- 途径
- Hedgehog Signaling, Chromatin Binding
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