Peptide ELISA: Limit dilution 1: 128000. Western blot: 0.1-0.3 μg/mL. Approx 75 kDa band observed in lysates of cell line HEK293(calculated MW of 62.5 kDa according to NP_005700.2). Immunohistochemistry: 10 μg/mL. In paraffin embedded Human Kidney, shows diffusecytopasmic staining in epithelial cells of renal tubules. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
0.5 mg/mL
缓冲液
Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumi
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.