KLF15 抗体 (N-Term)
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- 抗原 See all KLF15 抗体
- KLF15 (Kruppel-Like Factor 15 (KLF15))
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抗原表位
- N-Term
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适用
- 人, 小鼠, 大鼠, Pig
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This KLF15 antibody is un-conjugated
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应用范围
- Western Blotting (WB)
- 序列
- VDHLLPVDEN FSS-C
- 特异性
- This antibody detects KLF15 at N-term.
- 交叉反应 （详细）
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Species reactivity (expected):Mouse, Rat, Pig.
Species reactivity (tested):Human. - 纯化方法
- Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide
- 免疫原
- Peptide from N Terminus of the protein sequence according to NP_054798.1 Genename: KLF15
- Top Product
- Discover our top product KLF15 Primary Antibody
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anti-Kruppel-Like Factor 15 (KLF15) (AA 1-78) antibody
KLF15 适用: 人 WB, ELISA, IP 宿主: 小鼠 Monoclonal 1F3 unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (N-Term) antibodyVerified KLF15 适用: 人 WB, ELISA, FACS 宿主: 山羊 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 1-364) antibodyKLF15 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 1-260) antibodyKLF15 适用: 人 ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 1-416) antibodyKLF15 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 1-416) antibodyKLF15 适用: 人 WB, IF 宿主: 小鼠 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 45-94) antibodyKLF15 适用: 人, 小鼠, 大鼠, 犬, 豚鼠, Cow, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (AA 45-94) antibodyKLF15 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) (N-Term) antibodyKLF15 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Kruppel-Like Factor 15 (KLF15) antibodyKLF15 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 2G8 unconjugated
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- 应用备注
- Peptide ELISA: Limit dilution 1: 32000. Western blot: 0.3-1.0 μg/mL. Approx 48 kDa band observed in lysates of hepatoblastomacell line HepG2 (calculated MW of 44.8 kDa according to NP_054798.1). Recommended(Data obtained with a previous EB05463 batch). Identical WB results were obtained with
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C.
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- 抗原
- KLF15 (Kruppel-Like Factor 15 (KLF15))
- 别名
- KLF15 (KLF15 产品)
- 别名
- KKLF antibody, 1810013I09Rik antibody, AV048136 antibody, AW494632 antibody, CKLF antibody, Kruppel like factor 15 antibody, Kruppel-like factor 15 S homeolog antibody, Kruppel-like factor 15 antibody, KLF15 antibody, klf15.S antibody, Klf15 antibody
- 背景
- The kidney chloride channel proteins CLCNKA and CLCNKB are highly related, although they are located in different areas of the kidney. Using a yeast 1-hybrid screen of a kidney cDNA library with the GA element of the CLCNKA promoter as bait, Uchida et al. (2000) isolated cDNAs encoding MAZ and KLF15, which they termed KKLF. Sequence analysis predicted that the 415-amino acid KLF15 protein, which is 84 % identical to the rat Klf15 protein, contains 3 zinc finger motifs at its C terminus, N-terminal serine-rich stretches, and a central proline-rich segment. EMSA analysis confirmed that KLF15 and MAZ interact with the GA element of the CLCNKA promoter and showed that KLF15 binds with higher affinity and is a functional competitor of MAZ. Northern blot analysis revealed highest expression of a 2.5-kb KLF15 transcript in liver, followed by heart, skeletal muscle, and kidney. No expression was found in bone marrow or lymphoid tissues. Western blot analysis showed expression of a 50-kD protein. Immunohistochemical analysis detected nuclear expression of KLF15 in liver sinusoid stellate cells, subcapsular fibroblasts, and portal fibroblasts. KLF15 was also expressed in cardiac and skeletal muscle interstitial cells and in kidney inner medulla, glomeruli, and cortical interstitium. Immunofluorescence microscopy, however, demonstrated no colocalization of KLF15 with CLCNKA.Synonyms: CKLF, Cardiovascular Krueppel-like factor, KKLF, KLF15, Kidney-enriched krueppel-like factor, Krueppel-like factor 15
- 基因ID
- 28999
- NCBI登录号
- NP_054798
- UniProt
- Q9UIH9
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