SET/TAF-I 抗体 (N-Term)
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Quick Overview for SET/TAF-I 抗体 (N-Term) (ABIN374103)
抗原
See all SET/TAF-I (SET) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- N-Term
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序列
- SAPAAKVSKK ELNS-C
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特异性
- This antibody detects SET / I2PP2A at N-term.
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交叉反应 （详细）
- Species reactivity (expected):Bovine (Cow)Species reactivity (tested):Human.
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纯化方法
- Ammonium Sulphate Precipitation followed by antigen Affinity Chromatography using the immunizing peptide
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免疫原
- Peptide from the N Terminus of the protein sequence according to NP_003002.2
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anti-SET Nuclear Oncogene (SET) antibody
SET 适用: 人 WB, IHC, IF, IP 宿主: 兔 Monoclonal unconjugated
anti-SET Nuclear Oncogene (SET) (N-Term) antibodyVerified SET 适用: 人 WB, IHC, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) antibodySET 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) antibodySET 适用: 人 WB, FACS, ICC 宿主: 兔 Monoclonal 24GB1530 unconjugated Recombinant Antibody
anti-SET Nuclear Oncogene (SET) (AA 1-277) antibodySET 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal M1-F5 unconjugated
anti-SET Nuclear Oncogene (SET) (N-Term) antibodySET 适用: 人 WB, IF, ICC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) antibodySET 适用: 人, 小鼠, 大鼠 WB, FACS, ICC 宿主: 兔 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) (AA 147-235) antibodySET 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) (AA 35-84) antibodySET 适用: 人, 小鼠, 大鼠, 小鸡, 非洲爪蟾, 斑马鱼, Cow, 马, 豚鼠, 兔, 猴, Pig WB 宿主: 兔 Polyclonal unconjugated
anti-SET Nuclear Oncogene (SET) (N-Term) antibodySET 适用: 人, 小鼠, 大鼠 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- Peptide ELISA: Limit Dilution 1/1000. Western blot: 1-3 μg/mL. Approx 20-25 kDa and 45 kDa bands seen in Human Kidney
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限制
- 仅限研究用
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浓度
- 0.5 mg/mL
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缓冲液
- Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- SET/TAF-I (SET) (SET Nuclear Oncogene (SET))
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别名
- SET / I2PP2A
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背景
- SET belongs to a family of multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone binding. There are two named isoforms produced by alternative splicing: Isoform 1 and Isoform 2. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins, however, isoform 2 specific activity is higher. Isoform 1 and isoform 2 interact directly with each other and with ANP32A within the tripartite INHAT (inhibitor of acetyltransferases) complex. A chromosomal aberration involving SET is found in some cases of acute undifferentiated leukemia (AUL). Translocation t(6,9)(q21,q34.1) with NUP214/CAN.Synonyms: I-2PP2A, IGAAD, Inhibitor of granzyme A-activated DNase, PHAPII, Phosphatase 2A inhibitor I2PP2A, TAF-I, Template-activating factor I
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基因ID
- 6418
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NCBI登录号
- NP_003002
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UniProt
- H0UI37
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途径
- Apoptosis
抗原
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