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p53 抗体 (AA 4-8)

Name: p53 抗体 (AA 4-8)
SKU: ABIN362642
Availability: OutOfStock

This anti-p53 antibody is a 兔多克隆 antibody detecting p53 in WB. Suitable for 人和小鼠. This Primary Antibody has been cited in 2+ publications.

(2 references)

产品编号 ABIN362642

发货至: 中国

Quick Overview for p53 抗体 (AA 4-8) (ABIN362642)

抗原

See all p53 (TP53) 抗体

p53 (TP53) (Tumor Protein P53 (TP53))

适用

人, 小鼠

宿主

兔

克隆类型

多克隆

标记

This p53 antibody is un-conjugated

应用范围

Western Blotting (WB)

Discover our top product TP53 Primary Antibody

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AA 4-8

特异性

The antibody detects endogenous level of total p53 protein.

纯化方法

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

免疫原

Peptide sequence around AA 4-8 (P-Q-S-D-P) derived from Human p53. Antibodies were produced by immunizing rabbits with synthetic peptide and KLH conjugates.

亚型

IgG
anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 1-100) antibody
TP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) antibody
KO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (N-Term) antibody
TP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 301-393) antibody
TP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (pSer33) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Tumor Protein P53 (TP53) (pSer20) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (pSer15) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blotting: 1:500-1:1000

限制

仅限研究用
状态

Liquid

浓度

1 mg/mL

缓冲液

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Ji, Ding, Hawke, Xing, Jiang, Mills, Lu: "AKT-dependent phosphorylation of Niban regulates nucleophosmin- and MDM2-mediated p53 stability and cell apoptosis." in: EMBO reports, Vol. 13, Issue 6, pp. 554-60, (2012) (PubMed).

Gao, Zhai, Guan, Zheng: "Nordihydroguaiaretic acid inhibits growth of cervical cancer SiHa cells by up-regulating p21." in: Oncology letters, Vol. 2, Issue 1, pp. 123-128, (2012) (PubMed).
抗原

p53 (TP53) (Tumor Protein P53 (TP53))

别名

p53

背景

P53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes

分子量

53 kDa

NCBI登录号

NP_000537

UniProt

P04637

途径

p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect