BRAF 抗体 (pThr439)
Quick Overview for BRAF 抗体 (pThr439) (ABIN358271)
抗原
See all BRAF 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pThr439
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特异性
- This antibody detects BRAF pThr439.
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纯化方法
- Protein A Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with peptides. The antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding Thr439 of human BRAF.
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亚型
- Ig Fraction
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anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibody
BRAF 适用: 人, 犬 WB, IHC, IHC (p) 宿主: 小鼠 Monoclonal 4C11 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 大鼠, 猴, 犬 WB, IHC, IF, IHC (p) 宿主: 小鼠 Monoclonal 3A7 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 猴 WB, IHC, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 5A9 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 346-445) antibodyBRAF 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3D2 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人 ELISA, IHC 宿主: 小鼠 Monoclonal 1H12F1 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) (AA 424-453) antibodyBRAF 适用: 人 WB, ELISA, IHC, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 1H12 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, IF 宿主: 小鼠 Monoclonal 5C5 unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 人, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-B-Raf proto-oncogene, serine/threonine kinase (BRAF) antibodyBRAF 适用: 小鼠, 大鼠 IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- BRAF (B-Raf proto-oncogene, serine/threonine kinase (BRAF))
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背景
- BRAF is involved in the transduction of mitogenic signals from the cell membrane to the nucleus. It may play a role in the postsynaptic responses of hippocampal neuron. Defects in BRAF are a cause of cardiofaciocutaneous syndrome (CFC syndrome), also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.Synonyms: B-Raf proto-oncogene serine/threonine-protein kinase, BRAF, BRAF1, RAFB1, p94, v-Raf murine sarcoma viral oncogene homolog B1
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分子量
- 84437 Da
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基因ID
- 673, 9606
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UniProt
- P15056
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途径
- MAPK Pathway, RTK signaling, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Hepatitis C, Autophagy
抗原
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