Retinoblastoma 1 抗体 (pSer811)
Quick Overview for Retinoblastoma 1 抗体 (pSer811) (ABIN358217)
抗原
See all Retinoblastoma 1 (RB1) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- pSer811
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特异性
- This antibody detects Rb pSer811. Predicted to cross react with Mouse (100 % Antigen Homology).
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纯化方法
- Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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免疫原
- This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S811 of human RB.
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亚型
- Ig Fraction
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应用备注
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ELISA: 1/1,000. Dot Blot: 1/500. Immunohistochemistry: 1/50approx. 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.25 mg/mL
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缓冲液
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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储存液
- Sodium azide
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注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C/-20 °C
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储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- Retinoblastoma 1 (RB1)
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别名
- Retinoblastoma-Associated Protein / RB1
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背景
- Retinoblastoma (RB) is an embryonic malignant neoplasm of retinal origin. It almost always presents in early childhood and is often bilateral. Spontaneous regression occurs in some cases.Synonyms: OSRC, Rb, Retinoblastoma 1, p105-Rb, pRb, pp110
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分子量
- 106159 Da.
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基因ID
- 5925, 9606
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UniProt
- P06400
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途径
- Cell Division Cycle, Intracellular Steroid Hormone Receptor Signaling Pathway, Mitotic G1-G1/S Phases, DNA Replication, Maintenance of Protein Location, Synthesis of DNA, Autophagy
抗原
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