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RPS6KA1 抗体 (C-Term, pSer732)

Name: RPS6KA1 抗体 (C-Term, pSer732)
Brand: antibodies-online
SKU: ABIN349634
Availability: OutOfStock

RPS6KA1 适用: 人, 小鼠, 大鼠, Cow, 犬, 马 WB, ELISA 宿主: 兔 Polyclonal unconjugated

Western blot using affinity purified anti-p90 RSK1 pS732 antibody. (RPS6KA1 抗体 (C-Term, pSer732))

Western blot using affinity purified anti-p90 RSK1 pS732 antibody shows detection of a band ~90 kDa in size corresponding to phosphorylated p90 RSK1 (arrowhead) in EGF stimulated (lane 2) HEK293T cell lysates prepared from cells grown in the absence of serum for 12 h. (RPS6KA1 抗体 (C-Term, pSer732))

2 images

产品编号 ABIN349634

发货至: 中国

Datasheet as PDF

抗原 See all RPS6KA1 抗体

RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))
抗原表位
39

28

24

17

16

15

13

8

7

6

6

5

5

3

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
C-Term, pSer732
适用
196

121

89

6

4

4

4

3

3

3

2

2

1

1

1

1
人, 小鼠, 大鼠, Cow, 犬, 马
宿主
206

17
兔
Compatible Secondaries
克隆类型
186

38
多克隆
标记
100

17

14

11

8

8

8

8

8

8

7

7

4

4

4

4

4
This RPS6KA1 antibody is un-conjugated
应用范围
175

100

54

53

50

24

20

20

12

9

9

3

2

1
Western Blotting (WB), ELISA

特异性

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography using phosphorylated peptide coupled to agarose beads followed by solid phase adsorption against non phosphorylated peptide. This antibody is specific for human p90 RSK1 protein phosphorylated at S732. A BLAST analysis was used to suggest cross reactivity with p90 RSK1 from human, rat, mouse, horse, bovine, and dog based on 100% homology with the immunizing sequence. Partially cross reactivity may occur against opossum and chicken based on >90% homology with the immunizing sequence. Cross reactivity with p90 RSK1 pS732 from other sources has not been determined.

交叉反应

小鼠, 大鼠, 马, 绵羊, 犬

产品特性

Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.

纯化方法

affinity purified

过滤

Sterile filtered

免疫原

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the C-terminal end of human RSK1 protein.
Immunogen Type: Peptide

亚型

IgG

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anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 62-321) antibody
RPS6KA1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 1-735) antibody
RPS6KA1 适用: 人 WB, ELISA, IF 宿主: 小鼠 Monoclonal 4E11 unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (C-Term) antibody
RPS6KA1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (Thr573) antibody
RPS6KA1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibody
RPS6KA1 适用: 人 WB, ELISA, IP, IF 宿主: 兔 Monoclonal 1B6 unconjugated Recombinant Antibody

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pThr348) antibody
RPS6KA1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) antibody
RPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (pSer363), (pThr359) antibody
RPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (AA 539-588) antibody
RPS6KA1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1) (C-Term) antibody
RPS6KA1 适用: 人 WB, ELISA, IP, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

This affinity-purified antibody has been tested for use in ELISA and western blotting. Specific conditions for reactivity should be optimized by the end user. By western blot a band approximately 90 kDa in size corresponding to p90 RSK1 pS732 protein is expected in the appropriate cell lysate or extract.

说明

Gene Name: RPS6KA1

限制

仅限研究用
状态

Liquid

浓度

1.7 mg/mL

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C/-20 °C

储存方法

Store vial at 4 °C prior to restoration. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4 °C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is three (3) months from date of opening.

有效期

3 months
抗原

RPS6KA1 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 1 (RPS6KA1))

别名

p90 RSK1 (RPS6KA1 产品)

别名

HU-1 antibody, MAPKAPK1A antibody, RSK antibody, RSK1 antibody, Rsk1 antibody, p90rsk antibody, rsk antibody, RPS6KA antibody, hu-1 antibody, mapkapk1a antibody, rsk1 antibody, wu:fc56h07 antibody, zgc:152654 antibody, ribosomal protein S6 kinase A1 antibody, ribosomal protein S6 kinase polypeptide 1 antibody, ribosomal protein S6 kinase A1-like antibody, ribosomal protein S6 kinase A1 L homeolog antibody, ribosomal protein S6 kinase a, polypeptide 1 antibody, RPS6KA1 antibody, Rps6ka1 antibody, RPS6KA1L antibody, rps6ka1.L antibody, rps6ka1 antibody

背景

Ribosomal S6 Kinase 1 (RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A) is an immediate downstream effector of mitogen activated protein kinases and therefore promotes cell proliferation and survival. It has serine/threonine kinase activity and may play a role in mediating the growth-factor and stress-induced activation of the transcription factor CREB. The C-terminal region of RSK1 is reported to be an ERK docking site, where serine 732 phosphorylation status is critical for RSK1 activation. When serine 732 is not phosphorylated, ERK1/2 binds to the ERK docking site of RSK1, and upon stimulation, activates RSK1. The activated RSK1 then autophosphorylates serine 732, leading to the dissociation of ERK from RSK1 and termination of activation by ERK. RSK1 is studied in cancer research and is known to inactive tumor supressor complexes and death kinases.
Synonyms: p90 RSK1, Ribosomal S6 Kinase 1, RSK1, S6K-alpha 1, 90 kDa Ribosomal Protein S6 Kinase 1, MAP kinase-activated protein kinase 1a, or MAPKAPK1A

基因ID

6195

NCBI登录号

NP_001006666

UniProt

Q15418

途径

MAPK Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades