Insulin 抗体
Quick Overview for Insulin 抗体 (ABIN337249)
抗原
See all Insulin (INS) 抗体适用
宿主
克隆类型
标记
应用范围
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品牌
- IHC-plus™
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特异性
- Recognizes endogenous levels of total human insulin protein.
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纯化方法
- Immunoaffinity purified
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免疫原
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Synthetic peptide (KLH-coupled) derived from the sequence of human insulin.
Type of Immunogen: Synthetic peptide -
亚型
- IgG
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应用备注
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Approved: Flo, ICC, IF, IHC, IHC-P (1:100)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 1:100. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- 10 mM HEPES, pH 7.5, 150 mM sodium chloride, 100 μg/mL BSA, 50 % glycerol.
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注意事项
- Avoid repeat freeze-thaw cycles.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: 4°C. Long term: Store at -20°C. Avoid freeze-thaw cycles.
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- Insulin (INS)
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别名
- Insulin
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背景
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Name/Gene ID: INS
Family: Hormone
Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin -
基因ID
- 3630
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UniProt
- P01308
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
抗原
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