Insulin 抗体 (HRP)
Quick Overview for Insulin 抗体 (HRP) (ABIN337134)
抗原
See all Insulin (INS) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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特异性
- Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30 % ) and porcine insulin. Does not cross-react with free C-peptide.
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纯化方法
- Protein A purified
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免疫原
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Purified human insulin.
Type of Immunogen: Purified protein -
亚型
- IgG1 kappa
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应用备注
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Approved: ELISA, IHC, IHC-P (10 μg/mL)
Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. -
说明
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Target Species of Antibody: Human
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- Lot specific
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缓冲液
- PBS, pH 7.4, 0.05 % Proclin 300
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储存液
- ProClin
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注意事项
- This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid freeze-thaw cycles and prolonged exposure to light.
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储存条件
- 4 °C,-20 °C
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储存方法
- Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
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- Insulin (INS)
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别名
- Insulin
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背景
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Name/Gene ID: INS
Family: Hormone
Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin -
基因ID
- 3630
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UniProt
- P01308
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途径
- NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
抗原
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