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Insulin 抗体 (HRP)

This anti-Insulin antibody is a 小鼠 单克隆 antibody detecting Insulin in IHC, ELISA 和 IHC (p). Suitable for 人.
产品编号 ABIN337134
发货至: 中国

Quick Overview for Insulin 抗体 (HRP) (ABIN337134)

抗原

See all Insulin (INS) 抗体
Insulin (INS)

适用

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宿主

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小鼠

克隆类型

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单克隆

标记

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This Insulin antibody is conjugated to HRP

应用范围

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Immunohistochemistry (IHC), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

克隆位点

8E2
  • 特异性

    Recognizes human Insulin. Species cross-reactivity: human proinsulin, bovine insulin (30 % ) and porcine insulin. Does not cross-react with free C-peptide.

    纯化方法

    Protein A purified

    免疫原

    Purified human insulin.

    Type of Immunogen: Purified protein

    亚型

    IgG1 kappa
  • 应用备注

    Approved: ELISA, IHC, IHC-P (10 μg/mL)

    Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in  pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Recommended as a detection antibody for ELISA and in sandwich assay with clone 3A6 as the capture antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

    说明

    Target Species of Antibody: Human

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    Lot specific

    缓冲液

    PBS,  pH 7.4, 0.05 % Proclin 300

    储存液

    ProClin

    注意事项

    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    注意事项

    Avoid freeze-thaw cycles and prolonged exposure to light.

    储存条件

    4 °C,-20 °C

    储存方法

    Short term: store at 4°C. Long term: aliquot and store -20°C. Avoid freeze-thaw cycles. Protect from light.
  • 抗原

    Insulin (INS)

    别名

    Insulin

    背景

    Name/Gene ID: INS
    Family: Hormone

    Synonyms: INS, IRDN, IDDM2, Preproinsulin, ILPR, Insulin, MODY10, Proinsulin

    基因ID

    3630

    UniProt

    P01308

    途径

    NF-kappaB Signaling, RTK signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation
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