PDGF-BB Homodimer (AA 82-190) 抗体
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- 抗原
- PDGF-BB Homodimer
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抗原表位
- AA 82-190
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunocytochemistry (ICC)
- 原理
- Monoclonal Antibody to Platelet Derived Growth Factor BB (PDGF BB)
- 序列
- MHHHHHHSSG LVPRGSGMKE TAAAKFERQH MDSPDLGTDD DDKAMADIGS EF- SLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
- 特异性
- The antibody is a mouse monoclonal antibody raised against PDGF BB. It has been selected for its ability to recognize PDGF BB in immunohistochemical staining and western blotting.
- 交叉反应
- 小鼠, 大鼠
- 纯化方法
- Protein A + Protein G affinity chromatography
- 免疫原
- Recombinant Platelet Derived Growth Factor BB (PDGF BB) corresdonding to Ser82~Thr190 with N-terminal His Tag
- 克隆位点
- 4#
- 亚型
- IgG2b kappa
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- 应用备注
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Western blotting: 0.5-2 μg/mL
1:500-2000 Immunohistochemistry: 5-20 μg/mL
1:50-200 Immunocytochemistry: 5-20 μg/mL
1:50-200 Optimal working dilutions must be determined by end user.
- 说明
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The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- PBS, pH 7.4, containing 0.02 % Sodium azide, 50 % glycerol.
- 储存液
- Sodium azide
- 注意事项
- WARNING: Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azide-containing compounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing.
- 注意事项
- Avoid repeated freeze/thaw cycles
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store at 4°C for frequent use. Stored at -20°C in a manual defrost freezer for two year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
- 有效期
- 24 months
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- 抗原
- PDGF-BB Homodimer
- 别名
- Platelet Derived Growth Factor BB
- 别名
- PDGF-2 antibody, PDGF2 antibody, SIS antibody, SSV antibody, c-sis antibody, PDGF-B antibody, Sis antibody, platelet derived growth factor subunit B antibody, platelet derived growth factor, B polypeptide antibody, PDGFB antibody, Pdgfb antibody
- 背景
- PDGF-BB
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