p53 抗体 (pSer46)
(3 references)Quick Overview for p53 抗体 (pSer46) (ABIN3201005)
抗原
See all p53 (TP53) 抗体适用
宿主
克隆类型
标记
应用范围
克隆位点
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抗原表位
- pSer46
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交叉反应 （详细）
- Not tested in other species.
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纯化方法
- Purified from serum-free culture medium of hybridoma (#36) by proprietary chromatography procedures under mild conditions.
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过滤
- Sterile filtered
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免疫原
- Synthetic peptide containing pSer46 of p53
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亚型
- IgG1 kappa
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anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 1-100) antibodyTP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) antibodyKO Validated TP53 适用: 人 WB, IF, IHC (p), IP, ICC, ChIP 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (N-Term) antibodyTP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 301-393) antibodyTP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer33) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated
anti-Tumor Protein P53 (TP53) (AA 94-201) antibodyTP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated
anti-Tumor Protein P53 (TP53) (pSer20) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Tumor Protein P53 (TP53) (pSer15) antibodyTP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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1) Western blotting: 1,000~2,000 dilution
2) Immunohistochemistry (assay dependent)
3) ELISA
Other applications have not been tested. -
限制
- 仅限研究用
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状态
- Liquid
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浓度
- 1 mg/mL
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缓冲液
- PBS (x1), 50 % glycerol. Azide and carrier free.
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储存液
- Azide free
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储存条件
- -20 °C
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储存方法
- Upon arrival centrifuge briefly and store at -20 C.
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: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, (2010) (PubMed).
: "Requirement of ATM for rapid p53 phosphorylation at Ser46 without Ser/Thr-Gln sequences." in: Molecular and cellular biology, Vol. 30, Issue 7, pp. 1620-33, (2010) (PubMed).
: "DYRK2 is targeted to the nucleus and controls p53 via Ser46 phosphorylation in the apoptotic response to DNA damage." in: Molecular cell, Vol. 25, Issue 5, pp. 725-38, (2007) (PubMed).
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: "ATM augments nuclear stabilization of DYRK2 by inhibiting MDM2 in the apoptotic response to DNA damage." in: The Journal of biological chemistry, Vol. 285, Issue 7, pp. 4909-19, (2010) (PubMed).
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- p53 (TP53) (Tumor Protein P53 (TP53))
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别名
- p53
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背景
- P53 mutants are found in more than half of human cancers and are considered as the most important human cancer related gene. p53 is detected at 53kD position by electrophoresis and is composed of 393 amino acids. In the unstressed normal cells the p53 level is low and it is inactive. However, with stress, especially with DNA damage, it is activated to promote arrest of cell cycle and repair of DNA damage, or induction of apoptosis. The functions of p53 are regulated by phosphorylation of serine and threonine, and acetylation of lysine at various sites in the molecule. Among the phosphorylation sites, Ser46 is phosphorylated when DNA damage is so severe as to become unrepairable, which leads to apoptosis by activating transcription of proapoptotic genes such as p53AIPI As to the kinase of phosphorylation of Ser46, involvement of DYRK2 and ATM have been implicated.
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UniProt
- P04637
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途径
- p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
抗原
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