CD80 抗体
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- 抗原 See all CD80 抗体
- CD80
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This CD80 antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 特异性
- This antibody recognises CD80 (B7-1), a 60 kDa cell surface glycoprotein which is a member of the CD28/B7 family. In mice, CD80 is expressed on monocytes, peritoneal macrophages and dendritic cells. Expression may be significantly increased upon B lymphocytes by LPS and by IL-4.
- 产品特性
- Synonyms: CD28LG, CD28LG1, LAB7, BB1, Activation B7-1 antigen, B7.1, T-lymphocyte activationantigen CD80, CTLA-4 counter-receptor B7.1
- 纯化方法
- Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant
- 免疫原
- BCL1 cells expressing CD80
- 克隆位点
- RM80
- 亚型
- IgG2a
- Top Product
- Discover our top product CD80 Primary Antibody
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anti-CD80 (CD80) (AA 35-288) antibody
CD80 适用: 人 FACS, WB, ELISA, IHC, ICC, Neut 宿主: 小鼠 Monoclonal 1G3D7 unconjugated
anti-CD80 (CD80) (AA 251-321) antibodyCD80 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), ICC, IHC (fro), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated
anti-CD80 (CD80) (AA 38-246) antibodyCD80 适用: 小鼠 WB, IP, IHC, ICC 宿主: 兔 Polyclonal unconjugated
anti-CD80 (CD80) antibodyCD80 适用: 小鼠, 犬 FACS, IP, IHC (fro) 宿主: Armenian Hamster Monoclonal 16-10A1 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibodyCD80 适用: 人 FACS, IP 宿主: 小鼠 Monoclonal MEM-233 unconjugated
anti-CD80 (CD80) (Extracellular Domain) antibody (FITC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 FITC
anti-CD80 (CD80) (Extracellular Domain) antibody (PE)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PE
anti-CD80 (CD80) (Extracellular Domain) antibody (APC)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 APC
anti-CD80 (CD80) (Extracellular Domain) antibody (PerCP)CD80 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-233 PerCP
anti-CD80 (CD80) antibodyCD80 适用: 人 ELISA, IHC, ICC 宿主: 小鼠 Monoclonal 2A2 unconjugated
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- 应用备注
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Flow cytometry: 1/10 - 1/25, use 10 μL of the suggested working dilution to label 1x10^6cells in 100 μL. Immunohistochemistry on frozen sections. Functional assays.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- Phosphate buffered saline, pH 7.4 containing 0,09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - 有效期
- 12 months
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- 抗原
- CD80
- 别名
- CD80 (CD80 产品)
- 别名
- B7 antibody, B7-1 antibody, B7.1 antibody, BB1 antibody, CD28LG antibody, CD28LG1 antibody, LAB7 antibody, B71 antibody, Cd28l antibody, Ly-53 antibody, Ly53 antibody, MIC17 antibody, TSA1 antibody, CD80 molecule antibody, Cd80 molecule antibody, CD80 antigen antibody, CD80 antibody, Cd80 antibody
- 背景
- CD80 is a member of the Ig superfamily and serves as the ligand for two T cell molecules, CD28 and CTLA4. Interactions between CD28 and CD80 on activated B cells result in enhanced T cell activation. CD80 is rapidly induced on the surface of in vitro activated B cells, Epstein Barr Virus (EBV) transformed B cell lines, Burkitts lymphoma cell lines, freshly isolated follicular B lymphoma cells, T cells, and monocytes. It is also expressed at high levels in dendritic cells. It reacts weakly with a small proportion of non activated normal B cells and with HTLV1 infected T cells. CD80 does not react with peripheral monocytes, resting and activated normal T cells, T cell lines and T cell clones, nor with myelomonocytic cell lines.Synonyms: Activation B7-1 antigen, B7-1, B7.1, BB1, CD28LG, CD28LG1, CTLA-4 counter-receptor B7.1, LAB7, T-lymphocyte activation antigen CD80
- 基因ID
- 12519
- UniProt
- Q00609
- 途径
- TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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