MEK1 抗体 (C-Term)
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- 抗原 See all MEK1 (MAP2K1) 抗体
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
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抗原表位
- AA 353-367, C-Term
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适用
- 人, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This MEK1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC)
- 原理
- Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 1(MAP2K1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
- 序列
- KQLMVHAFIK RSDAE
- 交叉反应 (详细)
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Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
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Rabbit IgG polyclonal antibody for Dual specificity mitogen-activated protein kinase kinase 1(MAP2K1) detection. Tested with WB, IHC-P, ICC in Human,Mouse,Rat.
Gene Name: mitogen-activated protein kinase kinase 1
Protein Name: Dual specificity mitogen-activated protein kinase kinase 1(MAP kinase kinase 1/) - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MEK1(353-367aa KQLMVHAFIKRSDAE), identical to the related mouse and rat sequences.
- 亚型
- IgG
- Top Product
- Discover our top product MAP2K1 Primary Antibody
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- 应用备注
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WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
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At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
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- 抗原
- MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))
- 别名
- MAP2K1 (MAP2K1 产品)
- 背景
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Dual specificity mitogen-activated protein kinase kinase 1 is an enzyme that in humans is encoded by the MAP2K1 gene. The protein encoded by this gene is a member of the dual specificity protein kinase family, which acts as a mitogen-activated protein(MAP) kinase kinase. MAP kinases, also known as extracellular signal-regulated kinases(ERKs), act as an integration point for multiple biochemical signals. This protein kinase lies upstream of MAP kinases and stimulates the enzymatic activity of MAP kinases upon activation by a wide variety of extra- and intracellular signals. As an essential component of the MAP kinase signal transduction pathway, this kinase is involved in many cellular processes such as proliferation, differentiation, transcription regulation and development. Rampoldi et al.(1997) localized the MAP2K1 gene to 15q22.1-q22.33.
Synonyms: Dual specificity mitogen activated protein kinase kinase 1 antibody|Dual specificity mitogen-activated protein kinase kinase 1 antibody|ERK activator kinase 1 antibody|MAP kinase kinase 1 antibody|MAP kinase/Erk kinase 1 antibody|MAP2K1 antibody|MAPK/ERK kinase 1 antibody|MAPKK 1 antibody|MAPKK1 antibody|MEK 1 antibody|Mek1 antibody|MEKK1 antibody|Mitogen activated protein kinase kinase 1 antibody|MKK 1 antibody|MKK1 antibody|MP2K1_HUMAN antibody|PRKMK1 antibody|Protein kinase mitogen activated kinase 1(MAP kinase kinase 1) antibody|Protein kinase mitogen activated, kinase 1 antibody - UniProt
- Q02750
- 途径
- MAPK Pathway, RTK signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling
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