SHC1 抗体 (C-Term)
-
- 抗原 See all SHC1 抗体
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
-
抗原表位
- AA 424-440, C-Term
-
适用
- 人, 大鼠
-
宿主
- 兔
-
克隆类型
- 多克隆
-
标记
- This SHC1 antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunocytochemistry (ICC), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 原理
- Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
- 序列
- DPSYVNVQNL DKARQAV
- 交叉反应 （详细）
-
Predicted Cross Reactivity: mouse
No cross reactivity with other proteins.
Predicted Cross Reactivity: Species predicted to be fit for the product based on sequence similarities. - 产品特性
-
Rabbit IgG polyclonal antibody for SHC-transforming protein 1(SHC1) detection. Tested with WB, IHC-P, IHC-F, ICC in Human,Mouse,Rat.
Gene Name: SHC(Src homology 2 domain containing) transforming protein 1
Protein Name: SHC-transforming protein 1 - 纯化方法
- Immunogen affinity purified.
- 免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human SHC(424-440aa DPSYVNVQNLDKARQAV), different from the related rat and mouse sequences by two amino acids.
- 亚型
- IgG
- Top Product
- Discover our top product SHC1 Primary Antibody
-
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 171-280) antibody
SHC1 适用: 人 WB, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 3F4 unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 385-495) antibodySHC1 适用: 人, 小鼠 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 2F7C7 unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 536-564), (C-Term) antibodySHC1 适用: 人, 小鼠, 大鼠 WB, IHC (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (C-Term) antibodySHC1 适用: 人, 小鼠, 大鼠 WB, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 272-559) antibodySHC1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) antibodySHC1 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pTyr427) antibodySHC1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pTyr349) antibodySHC1 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (AA 171-280) antibodySHC1 适用: 人 WB, IHC, ELISA, IF, IHC (p), PLA 宿主: 小鼠 Monoclonal 3F4 unconjugated
anti-SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1) (pSer36) antibodySHC1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated
-
- 应用备注
-
WB: Concentration: 0.1-0.5 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, The detection limit for SHC1 is approximately 1 ng/lane under reducing conditions.
IHC-P: Concentration: 0.5-1 μg/mL, Tested Species: Human, Rat, Predicted Species: Mouse, Epitope Retrieval by Heat: Boiling the paraffin sections in 10 mM citrate buffer, pH 6.0, for 20 mins is required for the staining of formalin/paraffin sections.
IHC-F: Concentration: 0.5-1 μg/mL, Tested Species: Rat, Predicted Species: Human, Mouse
ICC: Concentration: 0.5-1 μg/mL, Tested Species: Human
Notes: Tested Species: Species with positive results. Predicted Species: Species predicted to be fit for the product based on sequence similarities. Other applications have not been tested. Optimal dilutions should be determined by end users. - 说明
-
Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P), IHC(F) and ICC.
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
- 浓度
- 500 μg/mL
- 缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
- 储存液
- Thimerosal (Merthiolate), Sodium azide
- 注意事项
- This product contains Sodium azide and Thimerosal (Merthiolate): POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
-
At -20°C for one year. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20 °C for a longer time. Avoid repeated freezing and thawing. - 有效期
- 12 months
-
- 抗原
- SHC1 (SHC (Src Homology 2 Domain Containing) Transforming Protein 1 (SHC1))
- 别名
- SHC1 (SHC1 产品)
- 别名
- SHC1 antibody, SHC antibody, SHCA antibody, P66shc antibody, si:dkey-246i14.4 antibody, wu:fc10h03 antibody, shc antibody, p66shc antibody, Shc antibody, ShcA antibody, p66 antibody, SHC adaptor protein 1 antibody, Shc1p antibody, squalene--hopene cyclase antibody, SHC (Src homology 2 domain containing) transforming protein 1 antibody, SHC (Src homology 2 domain containing) transforming protein 1 L homeolog antibody, src homology 2 domain-containing transforming protein C1 antibody, SHC1 antibody, shc1 antibody, Shc1 antibody, shc1.L antibody
- 背景
-
SHC1(SHC-transforming protein 1), also known as SHCA, is a protein that in humans is encoded by the SHC1 gene. SCOP classifies the 3D structure as belonging to the SH2 domain family. By Southern analysis of somatic cell hybrids followed by both isotopic and fluorescence in situ hybridization, Huebner et al.(1994) assigned the SHC1 gene to 1q21. Yulug et al.(1995) used fluorescence in situ hybridization to map the SHC1 gene to 1q21. By the same method, an SHC-related sequence(SHCL1) was mapped to 17q21-q22. By FISH analysis and direct sequencing of vectorette library PCR products, Harun et al.(1997) identified SHC1P1, a 3.2-kb processed pseudogene, in Xq12-q13.1. SHC1P1 is 85 % identical to mouse Shc p66. Reporter assays showed FKHRL1 transactivates CAT, suggesting a capacity to augment antioxidant scavenging. Nemoto and Finkel(2002) concluded that there is an important functional relationship between forkhead proteins(e.g., FKHRL1), SHC1, and intracellular oxidants, all of which are though to be involved in the aging process in worms and mammals.
Synonyms: FLJ26504 antibody|MGC98812 antibody|p46 antibody|p52 antibody|p52 SHC antibody|p52SHC antibody|p66 antibody|p66 SHC antibody|p66SHC antibody|SH2 domain protein C1 antibody|SHC(Src homology 2 domain containing) transforming protein 1 antibody|SHC 1 antibody|SHC A antibody|Shc antibody|SHC transforming protein 1 antibody|SHC transforming protein antibody|SHC-transforming protein 1 antibody|SHC-transforming protein 3 antibody|SHC-transforming protein A antibody|SHC1 antibody|SHC1_HUMAN antibody|SHCA antibody|Src homology 2 domain containing transforming protein C1 antibody|Src homology 2 domain-containing-transforming protein C1 antibody - UniProt
- P29353
- 途径
- RTK signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, ER-Nucleus Signaling, Signaling Events mediated by VEGFR1 and VEGFR2
-
