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SQSTM1 抗体 (N-Term)

Name: SQSTM1 抗体 (N-Term)
SKU: ABIN3044026
Availability: OutOfStock

This anti-SQSTM1 antibody is a 兔多克隆 antibody detecting SQSTM1 in WB, IHC, IF, ICC 和 FACS. Suitable for 人, 小鼠和大鼠. This Primary Antibody has been cited in 4+ publications.

Anti-SQSTM1/p62 antibody, Western blotting Lane 1: Rat Brain Tissue Lysate Lane 2: HELA Cell Lysate Lane 3: U87 Cell Lysate Lane 4: A549 Cell Lysate (SQSTM1 抗体 (N-Term))

Anti-SQSTM1/p62 antibody, IHC(P) IHC(P): Human Intestinal Cancer Tissue (SQSTM1 抗体 (N-Term))

IHC

Anti-SQSTM1/p62 antibody, ICC ICC: Hela Cell (SQSTM1 抗体 (N-Term))

IHC

3 images

(4 references)

产品编号 ABIN3044026

发货至: 中国

Quick Overview for SQSTM1 抗体 (N-Term) (ABIN3044026)

抗原

See all SQSTM1 抗体

SQSTM1 (Sequestosome 1 (SQSTM1))

适用

人, 小鼠, 大鼠

宿主

兔

克隆类型

多克隆

标记

This SQSTM1 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Discover our top product SQSTM1 Primary Antibody

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AA 91-110, N-Term

原理

Anti-SQSTM1/p62 Antibody

序列

KDDIFRIYIK EKKECRRDHR

交叉反应（详细）

No cross-reactivity with other proteins

产品特性

Anti-SQSTM1/p62 Antibody (ABIN3044026). Tested in Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. This is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications.

纯化方法

Immunogen affinity purified.

免疫原

A synthetic peptide corresponding to a sequence at the N-terminus of human SQSTM1, different from the related rat and mouse sequences by one amino acid.

亚型

IgG
anti-Sequestosome 1 (SQSTM1) antibody
KO Validated SQSTM1 适用: 人 WB, IF, ICC, IP, FACS, IHC (p), PLA 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 51-150) antibody
SQSTM1 适用: 人, 小鼠, 大鼠, Cow WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 1-440) antibody
SQSTM1 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 232-356) antibody
SQSTM1 适用: 人 WB, IHC, ELISA, FACS 宿主: 小鼠 Monoclonal 5H7E2 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 317-346) antibody
SQSTM1 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB4614 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 136-400) antibody
SQSTM1 适用: 人 WB, IHC, ICC, IP, FACS 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) (C-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC, ELISA 宿主: 兔 Polyclonal unconjugated

anti-Sequestosome 1 (SQSTM1) antibody
SQSTM1 适用: 人, 猴 WB, IHC, IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal 5A2 unconjugated

anti-Sequestosome 1 (SQSTM1) (AA 69-96), (N-Term) antibody
SQSTM1 适用: 人, 小鼠, 大鼠 WB, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot, 0.1-0.5 μg/mL, Human
Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Immunocytochemistry/Immunofluorescence, 2 μg/mL, Human
Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human

说明

Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P) and ICC.

限制

仅限研究用
状态

Lyophilized

溶解方式

Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.

浓度

500 μg/mL

缓冲液

Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.

储存液

Thimerosal (Merthiolate), Sodium azide

注意事项

This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C,-20 °C

储存方法

Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

有效期

12 months
Yu, Li, Wang, He, Ding, Zhang, Yu, Shi, Cui, Wang, Wang, Sun, Zhang, Du, Zhu: "Interferon regulatory factor-1 activates autophagy to aggravate hepatic ischemia-reperfusion injury via the P38/P62 pathway in mice." in: Scientific reports, Vol. 7, pp. 43684, (2018) (PubMed).

Jeong, He, Nohara, Park, Shin, Kim, Shimomura, Koike, Yoo, Chen: "Dual attenuation of proteasomal and autophagic BMAL1 degradation in Clock Δ19/+ mice contributes to improved glucose homeostasis." in: Scientific reports, Vol. 5, pp. 12801, (2017) (PubMed).

He, Wang, Han, Jin, Li, Wu, Ma, Cheng, Tang, Yang, Liu: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).

Li, Jen, Wu, Lee, Fang, Quigley, Lee, Wang, Zhou, Vergnes, Chen, Li, Reue, Ann, Hsiai: "Disturbed Flow Induces Autophagy, but Impairs Autophagic Flux to Perturb Mitochondrial Homeostasis." in: Antioxidants & redox signaling, Vol. 23, Issue 15, pp. 1207-19, (2016) (PubMed).
抗原

SQSTM1 (Sequestosome 1 (SQSTM1))

别名

SQSTM1

背景

Synonyms: Sequestosome-1,EBI3-associated protein of 60 kDa,EBIAP,p60,Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa,Ubiquitin-binding protein p62,SQSTM1,ORCA, OSIL,
Tissue Specificity: Ubiquitously expressed. .
Background: SQSTM1 (Sequestosome-1), also known as Ubiquitin-Binding Protein P62 or P62, is a protein that in humans is encoded by the SQSTM1 gene. The Src homology type 2 (SH2) domain is a highly conserved motif of about 100 amino acids which mediates protein-protein interactions by binding to phosphotyrosine.p56-lck, a T-cell-specific src family tyrosine kinase with an SH2 domain, is involved in T-cell signal transduction. The International Radiation Hybrid Mapping Consortium mapped the p62 gene to chromosome 5q35. Park et al. (1995) found that the p56-lck SH2 domain binds to p62 at the ser59 of p62 only when that serine is phosphorylated. Joung et al. (1996) expressed epitope-tagged p62 in Hela cells and showed that the expressed protein bound to the lck SH2 domain and that this binding was dependent on the N-terminal 50 amino acids of p62 but not on the tyrosine residue in this region.
Sequence Similarities: Contains 1 PB1 domain.

分子量

60 kDa

UniProt

Q13501

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Autophagy