MAP3K1 抗体 (C-Term)
Quick Overview for MAP3K1 抗体 (C-Term) (ABIN3044007)
抗原
See all MAP3K1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 1418-1432, C-Term
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原理
- Anti-MEKK1/MAP3K1 Antibody Picoband®
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序列
- PEVLRGQQYG RSCDV
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-MEKK1/MAP3K1 Antibody (ABIN3044007). Tested in IHC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human MEKK1, identical to the related rat and mouse sequences.
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1307-1509) antibody
MAP3K1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibodyMAP3K1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1211-1310) antibodyMAP3K1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1077-1176) antibodyMAP3K1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibodyMAP3K1 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 马, 斑马鱼, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1211-1310) antibodyMAP3K1 适用: 人 WB, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibodyMAP3K1 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human, Mouse, Rat
Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Juriloff, D. M., Harris, M. J., Mah, D. G. The open-eyelid mutation, lidgap-Gates, is an eight-exon deletion in the mouse Map3k1 gene. Genomics 85: 139-142, 2005. 2. Vinik, B. S., Kay, E. S., Fiedorek, F. T., Jr. Mapping of the MEK kinase gene (Mekk) to mouse chromosome 13 and human chromosome 5. Mammalian Genome 6: 782-783, 1995. 3. Xia, Y., Wu, Z., Su, B., Murray, B., Karin, M. JNKK1 organizes a MAP kinase module through specific and sequential interactions with upstream and downstream components mediated by its amino-terminal extension. Genes Dev. 12: 3369-3381, 1998. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal, 0.05 mg Sodium azide.
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储存液
- Thimerosal (Merthiolate), Sodium azide
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注意事项
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))
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别名
- MAP3K1
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背景
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Synonyms: Mitogen-activated protein kinase kinase kinase 1,2.7.11.25,MAPK/ERK kinase kinase 1,MEK kinase 1,MEKK 1,MAP3K1,MAPKKK1, MEKK, MEKK1,
Background: MAP3K1 (Mitogen-activated protein kinase kinase kinase 1), also known as MEKK1, MAPKKK1, MEK KINASE or MAP/ERK KINASE KINASE 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al. (1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse Map3k1 gene and the human MAP3K1 homolog. Using these allele-specific polymorphisms, they mapped the Map3k1 gene in an intersubspecific backcross to mouse chromosome 13. They mapped the human MAP3K1 gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al. (1998) showed that human MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1). MEKK1 ly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 by MEKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al. (1998) concluded that JNKK1 is the preferred MEKK1 substrate.
Sequence Similarities: Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
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分子量
- 164 kDa
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UniProt
- Q13233
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途径
- MAPK Pathway, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades
抗原
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