IKK alpha 抗体 (AA 411-745)
Quick Overview for IKK alpha 抗体 (AA 411-745) (ABIN3043812)
抗原
See all IKK alpha (CHUK) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 411-745
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原理
- Anti-IKK alpha/CHUK Antibody Picoband®
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-IKK alpha/CHUK Antibody Picoband® (ABIN3043812). Tested in IHC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- E.coli-derived human IKK alpha recombinant protein (Position: V411-E745). Human IKK alpha shares 98% amino acid (aa) sequence identity with mouse IKK alpha.
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亚型
- IgG
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anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (C-Term) antibody
CHUK 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3G12H9 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB2159 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (N-Term) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 551-650) antibodyCHUK 适用: 小鼠, 大鼠 ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
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应用备注
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Immunohistochemistry (Paraffin-embedded Section), 0.5-1 μg/mL, Human
Western blot, 0.1-0.5 μg/mL, Human
1. Mock BA, Connelly MA, McBride OW, Kozak CA, Marcu KB (May 1995). "CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19". Genomics27 (2): 348-51. 2. Häcker H, Karin M (October 2006). "Regulation and function of IKK and IKK-related kinases".Sci. STKE 2006 (357): re13. 3. Descargues P, Sil AK, Karin M (October 2008). "IKKα, a critical regulator of epidermal differentiation and a suppressor of skin cancer". EMBO J. 27 (20): 2639-47. 4. Zhu F, Park E, Liu B, Xia X, Fischer SM, Hu Y (February 2009). "Critical role of IkappaB kinase alpha in embryonic skin development and skin carcinogenesis". Histol. Histopathol. 24 (2): 265-71. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB, supported by ABIN921231 in IHC(P).
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Sodium azide.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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别名
- CHUK
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背景
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Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IKK-alpha,IkBKA,IkappaB kinase,2.7.11.10,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1,IKK1,Nuclear factor NF-kappa-B inhibitor kinase alpha,NFKBIKA,Transcription factor 16,TCF-16,CHUK,IKKA, TCF16,
Tissue Specificity: Widely expressed.
Background: IKKA, also known as conserved helix-loop-helix ubiquitous kinase (CHUK) or IKBKA, is a protein kinase that in humans is encoded by the CHUK gene. It is mapped to 10q24.31. This gene encodes a member of the serine/threonine protein kinase family. The encoded protein, a component of a cytokine-activated protein complex that is an inhibitor of the essential transcription factor NF-kappa-B complex, phosphorylates sites that trigger the degradation of the inhibitor via the ubiquination pathway, thereby activating the transcription factor. IKKA is part of the IκB kinase complex that plays an important role in regulating the NF-κB transcription factor. However, IKKA also has many additional cellular targets, and is thought to function independently of the NF-κB pathway to regulate epidermal differentiation.
Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
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分子量
- 85 kDa
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基因ID
- 1147
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UniProt
- O15111
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途径
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
抗原
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