IKK alpha 抗体 (C-Term)
Quick Overview for IKK alpha 抗体 (C-Term) (ABIN3043147)
抗原
See all IKK alpha (CHUK) 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 728-745, C-Term
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原理
- Anti-IKK alpha/CHUK Antibody Picoband®
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序列
- NEEQGNSMMN LDWSWLTE
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-IKK alpha/CHUK Antibody (ABIN3043147). Tested in Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence at the C-terminus of human IKK alpha.
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亚型
- IgG
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anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (C-Term) antibody
CHUK 适用: 人 WB, IHC, IF, IP 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pSer176), (pSer177) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, IHC (p) 宿主: 小鼠 Monoclonal 4B8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 646-745) antibodyCHUK 适用: 人 WB, ELISA, IF, PLA 宿主: 小鼠 Monoclonal 1F8 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人 WB, ELISA, ICC, FACS 宿主: 小鼠 Monoclonal 3G12H9 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (AA 411-745) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (pThr23) antibodyCHUK 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) antibodyCHUK 适用: 人, 小鼠 WB 宿主: 兔 Polyclonal RB2159 unconjugated
anti-conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK) (N-Term) antibodyCHUK 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human
Flow Cytometry(Fixed), 1-3 μg/1x106 cells, Human
1. Mock BA, Connelly MA, McBride OW, Kozak CA, Marcu KB (May 1995). "CHUK, a conserved helix-loop-helix ubiquitous kinase, maps to human chromosome 10 and mouse chromosome 19". 2. Anest, V., Hanson, J. L., Cogswell, P. C., Steinbrecher, K. A., Strahl, B. D., Baldwin, A. S. A nucleosomal function for I-kappa-B kinase-alpha in NF-kappa-B-dependent gene expression. Nature 423: 659-663, 2003. 3. Delhase, M., Hayakawa, M., Chen, Y., Karin, M. Positive and negative regulation of I-kappa-B kinase activity through IKK-beta subunit phosphorylation. Science 284: 309-312, 1999. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles. -
有效期
- 12 months
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- IKK alpha (CHUK) (conserved Helix-Loop-Helix Ubiquitous Kinase (CHUK))
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别名
- CHUK
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背景
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Synonyms: Inhibitor of nuclear factor kappa-B kinase subunit alpha,I-kappa-B kinase alpha,IKK-A,IKK-alpha,IkBKA,IkappaB kinase,2.7.11.10,Conserved helix-loop-helix ubiquitous kinase,I-kappa-B kinase 1,IKK1,Nuclear factor NF-kappa-B inhibitor kinase alpha,NFKBIKA,Transcription factor 16,TCF-16,CHUK,IKKA, TCF16,
Tissue Specificity: Widely expressed.
Background: IKBKA (INHIBITOR OF KAPPA LIGHT POLYPEPTIDE GENE ENHANCER IN B CELLS, KINASE OF, ALPHA), also called CHUK, NFKBIKA, IKKA, IKK1, I-KAPPA-B, is a protein kinase that in humans is encoded by the CHUK gene. And it is a member of the protein kinase superfamily. And the protein contains a serine/threonine kinase domain, due to its ubiquitous expression in a broad array of tissues and high degree of conservation across species. By FISH, the IKBKA gene is mapped to chromosome 10q24. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA or IKBKB) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NF-kappa-B complex. IKK-alpha is part of the I kappa B kinase complex that plays an important role in regulating the NF-kappa B transcription factor.
Sequence Similarities: Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. I-kappa-B kinase subfamily.
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分子量
- 85 kDa
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UniProt
- O15111
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途径
- PI3K-Akt Signaling, NF-kappaB Signaling, RTK signaling, TCR Signaling, TLR signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, Ubiquitin Proteasome Pathway, S100 Proteins
抗原
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