ATG5 抗体 (Middle Region)
(6 references)Quick Overview for ATG5 抗体 (Middle Region) (ABIN3042571)
抗原
See all ATG5 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- AA 82-97, Middle Region
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原理
- Anti-APG5L/ATG5 Antibody Picoband®
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序列
- DRFDQFWAIN RKLMEY
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交叉反应 （详细）
- No cross-reactivity with other proteins
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产品特性
- Anti-APG5L/ATG5 Antibody (ABIN3042571). Tested in WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.
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纯化方法
- Immunogen affinity purified.
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免疫原
- A synthetic peptide corresponding to a sequence in the middle region of human APG5L, identical to the related mouse sequence, and different from the related rat sequence by one amino acid.
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亚型
- IgG
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anti-ATG5 Autophagy Related 5 (ATG5) (AA 101-200) antibody
ATG5 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC (p), FACS, IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-275) antibodyATG5 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 209-238), (C-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p) 宿主: 兔 Polyclonal RB7547 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 144-275) antibodyATG5 适用: 人 ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 3C7A8 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 176-275) antibodyATG5 适用: 人 WB, ELISA 宿主: 小鼠 Monoclonal 1G11 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-119) antibodyATG5 适用: 人 WB, IHC, ELISA, Coat, StM 宿主: 小鼠 Monoclonal ATG5-2101 unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30) antibodyATG5 适用: 人, 小鼠 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 1-30), (N-Term) antibodyATG5 适用: 人, 小鼠 WB, IF, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (C-Term) antibodyATG5 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-ATG5 Autophagy Related 5 (ATG5) (AA 28-275) antibodyATG5 适用: 人 WB, IHC 宿主: 兔 Polyclonal unconjugated
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应用备注
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Western blot, 0.1-0.5 μg/mL, Human, Mouse, Rat
1. Baumann P, Cech TR (May 2001). "Pot1, the putative telomere end-binding protein in fission yeast and humans". Science 292 (5519): 1171-5. 2. Hammond EM, Brunet CL, Johnson GD, Parkhill J, Milner AE, Brady G, Gregory CD, Grand RJ (May 1998). "Homology between a human apoptosis specific protein and the product of APG5, a gene involved in autophagy in yeast". FEBS Lett 20 (3): 391-5. 3. Singh, R., Kaushik, S., Wang, Y., Xiang, Y., Novak, I., Komatsu, M., Tanaka, K., Cuervo, A. M., Czaja, M. J. Autophagy regulates lipid metabolism. Nature 458: 1131-1135, 2009. -
说明
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Antibody can be supported by chemiluminescence kit ABIN921124 in WB.
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限制
- 仅限研究用
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状态
- Lyophilized
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溶解方式
- Add 0.2 mL of distilled water will yield a concentration of 500 μg/mL.
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浓度
- 500 μg/mL
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缓冲液
- Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.
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注意事项
- Avoid repeated freezing and thawing.
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储存条件
- 4 °C,-20 °C
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储存方法
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Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month.
It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
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: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).
: "Nrf2 signalling and autophagy are involved in diabetes mellitus-induced defects in the development of mouse placenta." in: Open biology, Vol. 6, Issue 7, (2017) (PubMed).
: "Association between genetic polymorphisms in the autophagy-related 5 gene promoter and the risk of sepsis." in: Scientific reports, Vol. 7, Issue 1, pp. 9399, (2017) (PubMed).
: "Antagomirs Targeting MiroRNA-134 Attenuates Epilepsy in Rats through Regulation of Oxidative Stress, Mitochondrial Functions and Autophagy." in: Frontiers in pharmacology, Vol. 8, pp. 524, (2017) (PubMed).
: "Endocan silencing induces programmed cell death in hepatocarcinoma." in: Oncology letters, Vol. 14, Issue 5, pp. 5333-5339, (2017) (PubMed).
: "Anti-autophagic and anti-apoptotic effects of memantine in a SH-SY5Y cell model of Alzheimer's disease via mammalian target of rapamycin-dependent and -independent pathways." in: Molecular medicine reports, Vol. 12, Issue 5, pp. 7615-22, (2015) (PubMed).
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: "Proper autophagy is indispensable for angiogenesis during chick embryo development." in: Cell cycle (Georgetown, Tex.), Vol. 15, Issue 13, pp. 1742-54, (2017) (PubMed).
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- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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别名
- ATG5
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背景
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Synonyms: Autophagy protein 5,APG5-like,Apoptosis-specific protein,ATG5,APG5L, ASP,
Tissue Specificity: Ubiquitous. The mRNA is present at similar levels in viable and apoptotic cells, whereas the protein is dramatically highly expressed in apoptotic cells.
Background: Autophagy protein 5 is a protein that in humans is encoded by the ATG5 gene. It is also known as APG5 or ASP, and this gene is mapped to 6q21. It is found that knockdown of ATG5 in hepatocytes increased triglyceride levels with oleate or a second endogenous stimulus for triglyceride formation. These hepatocytes with ATG5 knockdown also had increased lipid droplet number and size. ATG5 is an E3 ubiquitin ligase which is necessary for autophagy due to its role in autophagosome elongation. It is activated by ATG7 and forms a complex with ATG12 and ATG16L1. This complex is necessary for LC3-1 conjugation to PE to form LC3-II.
Sequence Similarities: Belongs to the ubiquitin-conjugating enzyme family.
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分子量
- 55 kDa
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UniProt
- Q9H1Y0
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途径
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
抗原
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