MAP3K1 抗体 (C-Term)
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Quick Overview for MAP3K1 抗体 (C-Term) (ABIN3031846)
抗原
See all MAP3K1 抗体适用
宿主
克隆类型
标记
应用范围
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抗原表位
- C-Term
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纯化方法
- Antigen affinity
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免疫原
- An amino acid sequence from the C-terminus of human MEK kinase 1 (PEVLRGQQYGRSCDV) was used as the immunogen for this MAP3K1 antibody (100% homologous in human, mouse and rat).
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亚型
- IgG
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anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1307-1509) antibody
MAP3K1 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibodyMAP3K1 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1211-1310) antibodyMAP3K1 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1077-1176) antibodyMAP3K1 适用: 人 WB, IHC, StM 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (C-Term) antibodyMAP3K1 适用: 人, 小鼠, 大鼠, 兔, Cow, 豚鼠, 马, 斑马鱼, 犬 WB 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1211-1310) antibodyMAP3K1 适用: 人 WB, IHC (p), IF, FACS 宿主: 小鼠 Monoclonal 2F6 unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (pThr1402) antibodyMAP3K1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1) (AA 1251-1300) antibodyMAP3K1 适用: 人, 小鼠, 大鼠, 猴, 兔, Pig, Cow, 豚鼠, 马, 犬, Bat, 小鸡 WB 宿主: 兔 Polyclonal unconjugated
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应用备注
- The stated application concentrations are suggested starting amounts. Titration of the MAP3K1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
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限制
- 仅限研究用
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缓冲液
- 0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
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储存条件
- -20 °C
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储存方法
- After reconstitution, the MAP3K1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
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- MAP3K1 (Mitogen-Activated Protein Kinase Kinase Kinase 1 (MAP3K1))
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别名
- MAP3K1 (MEKK1)
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背景
- Mitogen-activated protein kinase kinase kinase 1, also known as MEKK1, MAPKKK1, MEK Kinase or MAP/ERK Kinase Kinase 1, is an enzyme that in humans is encoded by the MAP3K1 gene. Vinik et al.(1995) identified DNA sequence and size polymorphisms in intronic and 3-prime untranslated regions of the mouse gene and the human homolog. Using these allele-specific polymorphisms, they mapped the mouse gene in an intersubspecific backcross to chromosome 13. They mapped the human gene to chromosome 5 by somatic cell hybrid analysis. By assaying transfected COS-1 cells, Xia et al.(1998) showed that human MAP3K1 / MEKK1 activated JNK1 (MAPK8) robustly and p38-alpha (MAPK14) less efficiently, but it had only a marginal effect on ERK2 (MAPK1). MAP3K1 / MEKK1 directly and specifically interacted with JNKK1 (MAP2K4) and activated JNKK1 in cells and in vitro. Phosphorylation of JNKK1 disrupted their interaction. MEKK1 and JNK1 competed for binding to JNKK1. Xia et al.(1998) concluded that JNKK1 is the preferred MEKK1 substrate.
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UniProt
- Q13233
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途径
- MAPK Pathway, Interferon-gamma Pathway, Caspase Cascade in Apoptosis, TLR signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Regulation of Actin Filament Polymerization, Toll-Like Receptors Cascades
抗原
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