Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
纯化方法
Antigen affinity
免疫原
An amino acid sequence from the C-terminus of human Tuberous Sclerosis 1 (QLHIMDYNETHHEHS) was used as the immunogen for this TSC1 antibody (100% rat homology).
The stated application concentrations are suggested starting amounts. Titration of the TSC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.\. Western blot: 0.5-1 μg/mL,IHC (Paraffin): 0.5-1 μg/mL
限制
仅限研究用
缓冲液
0.5 mg/mL if reconstituted with 0.2 mL sterile DI water
储存条件
-20 °C
储存方法
After reconstitution, the TSC1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
TSC1 (Tuberous Sclerosis 1), also called Hamartin, is a human protein and gene. As part of a comprehensive strategy to identify the gene mutant in tuberous sclerosis-1, van Slegtenhorst et al.(1997) developed an overlapping contig of clones from the 1.4-Mb TSC1 region on chromosome 9. Benvenuto et al.(2000) showed that overexpression of the TSC1 gene in rat fibroblasts inhibits growth and causes changes in cell morphology. Van Slegtenhorst et al.(1998) showed that hamartin and tuberin associate physically in vivo, however, and that the interaction is mediated by predicted coiled-coil domains.