NAPSA 抗体
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- 抗原 See all NAPSA 抗体
- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
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适用
- 人
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This NAPSA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- A recombinant fragment from the human protein was used as the immunogen for the Napsin A antibody cocktail.
- 克隆位点
- NAPSA-1238-NAPSA-1239
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product NAPSA Primary Antibody
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- 应用备注
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Optimal dilution of the Napsin A antibody cocktail should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 min.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Western blot: 1-2 μg/mL,Immunohistochemistry (FFPE): 1-2 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Napsin A antibody cocktail at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- NAPSA (Napsin A Aspartic Peptidase (NAPSA))
- 别名
- Napsin A (NAPSA 产品)
- 别名
- KAP antibody, Kdap antibody, NAP1 antibody, NAPA antibody, SNAPA antibody, pronapsin antibody, kap antibody, kdap antibody, nap1 antibody, napa antibody, napsa antibody, snapa antibody, napsin A aspartic peptidase antibody, napsin A aspartic peptidase L homeolog antibody, NAPSA antibody, Napsa antibody, napsa.L antibody
- 背景
- Napsin is a pepsin-like aspartic proteinase connected with maturation of surfactant protein B. There are two closely related napsins, napsin A and napsin B. Napsin A is expressed as a single chain protein. Immunohistochemical studies revealed high expression levels of napsin A in human lung and kidney but low expression in spleen. Napsin A is expressed in type II pneumocytes and in adenocarcinomas of lung. The high specificity expression of napsin A in adenocarcinomas of lung is useful to distinguish primary lung adenocarcinomas from adenocarcinomas of other organs.
- 途径
- Tube Formation, Asymmetric Protein Localization, Embryonic Body Morphogenesis
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