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Cytokeratin 7 抗体

KRT7 适用: 人 IF, FACS, IHC (p) 宿主: 小鼠 Monoclonal KRT7-760 unconjugated
产品编号 ABIN3025125
发货至: 中国
  • 抗原 See all Cytokeratin 7 (KRT7) 抗体
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
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    小鼠
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    单克隆
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    This Cytokeratin 7 antibody is un-conjugated
    应用范围
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    Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    产品特性
    It recognizes an intermediate filament protein (IFP) of 55 kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
    纯化方法
    Protein G affinity chromatography
    免疫原
    Recombinant full-length human KRT7 protein was used as the immunogen for the Cytokeratin 7 antibody.
    克隆位点
    KRT7-760
    亚型
    IgG1
    Top Product
    Discover our top product KRT7 Primary Antibody
  • 应用备注
    Optimal dilution of the Cytokeratin 7 antibody should be determined by the researcher.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 0.5-1 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the Cytokeratin 7 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    Cytokeratin 7 (KRT7) (Keratin 7 (KRT7))
    别名
    Cytokeratin 7 (KRT7 产品)
    别名
    CK7 antibody, K2C7 antibody, K7 antibody, SCL antibody, D15Wsu77e antibody, Krt2-7 antibody, KRT7 antibody, keratin 7 antibody, KRT7 antibody, Krt7 antibody
    背景
    It recognizes an intermediate filament protein (IFP) of 55  kDa, which is identified as cytokeratin 7. This mAb is highly specific to cytokeratin 7 and shows no cross-reaction with other IFPs. Cytokeratin 7 is a basic cytokeratin, which is found in most glandular and transitional epithelia but not in the stratified squamous epithelia. Keratin 7 is expressed in the epithelial cells of ovary, lung, and breast but not of colon, prostate, or gastrointestinal tract. This mAb is highly useful in distinguishing ovarian carcinomas (keratin 7+) from colon carcinomas (keratin 7-).
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