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NGFR 抗体 (Extracellular Domain)

Name: NGFR 抗体 (Extracellular Domain)
SKU: ABIN3025050
Availability: OutOfStock

NGFR 适用: 人, 兔, Cat, 雪貂, 猴, Baboon FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal SPM299 unconjugated

Formalin-fixed, paraffin-embedded human melanoma stained with NGF Receptor antibody (SPM299). (NGFR 抗体 (Extracellular Domain))

IHC

1 image

产品编号 ABIN3025050

发货至: 中国

Datasheet as PDF

抗原 See all NGFR 抗体

NGFR (Nerve Growth Factor Receptor (NGFR))
抗原表位
16

13

9

8

6

6

5

4

4

4

4

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 1-160, Extracellular Domain
适用
158

59

55

28

26

26

25

20

10

5

4

4

3

3

2

2

2

1

1
人, 兔, Cat, 雪貂, 猴, Baboon
宿主
92

71

4

1
小鼠
Compatible Secondaries
克隆类型
88

80
单克隆
标记
88

15

10

7

6

4

3

3

3

3

3

3

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This NGFR antibody is un-conjugated
应用范围
103

79

56

55

45

27

27

19

18

14

14

12

9

6

4

3

3

2

1

1

1

1

1
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

无交叉反应

小鼠, 大鼠

产品特性

It recognizes a glycoprotein of 75 kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

纯化方法

Protein G affinity chromatography

免疫原

NGFR from A875 melanoma cells was used as the immunogen for the NGF Receptor antibody. Its epitope is within amino acids 1-160 of the extracellular domain of NGFR/NTR.

克隆位点

SPM299

亚型

IgG1 kappa

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anti-Nerve Growth Factor Receptor (NGFR) antibody
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anti-Nerve Growth Factor Receptor (NGFR) antibody
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anti-Nerve Growth Factor Receptor (NGFR) antibody
NGFR 适用: 人, 大鼠, 小鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated

应用备注

Optimal dilution of the NGF Receptor antibody should be determined by the researcher.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM Citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 min
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Flow Cytometry: 0.5-1 μg/million cells in 0.1ml,Immunofluorescence: 1-2 μg/mL,Immunohistochemistry (FFPE): 0.5-1 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2)

限制

仅限研究用
浓度

0.2 mg/mL

缓冲液

0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide

储存液

Sodium azide

注意事项

This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store the NGF Receptor antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
抗原

NGFR (Nerve Growth Factor Receptor (NGFR))

别名

NGF Receptor (NGFR 产品)

别名

ngfr antibody, wu:fb38c02 antibody, wu:fc10h01 antibody, CD271 antibody, Gp80-LNGFR antibody, TNFRSF16 antibody, p75(NTR) antibody, p75NTR antibody, LNGFR antibody, RNNGFRR antibody, Tnfrsf16 antibody, p75 antibody, NRH1b antibody, fullback antibody, nrh1 antibody, p75ntr antibody, tnfrsf16 antibody, p75NGFR antibody, nerve growth factor receptor antibody, neurotrophin receptor associated death domain antibody, nerve growth factor receptor S homeolog antibody, nerve growth factor receptor (TNFR superfamily, member 16) antibody, NGFR antibody, nradd antibody, Ngfr antibody, ngfr.S antibody

背景

It recognizes a glycoprotein of 75  kDa, identified as low affinity Nerve Growth Factor (NGF) Receptor (p75NGFR) or Neurotrophin Receptor (p75NTR). NGF-receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. NGF is important for the development, differentiation, and survival of variety of neuronal and non-neuronal cells. Its action is mediated by binding two distinct receptors, the high affinity p140 and low affinity p75.

途径

NF-kappaB Signaling, Neurotrophin Signaling Pathway, Carbohydrate Homeostasis, Growth Factor Binding