Smooth Muscle Actin 抗体 (N-Term)
-
- 抗原 See all Smooth Muscle Actin (ACTA2) 抗体
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
-
抗原表位
- N-Term
-
适用
- 人, 大鼠, 小鼠, 小鸡, Cow, 兔, 猴, Baboon, 豚鼠, 犬, Cat, Pig, 山羊, 绵羊
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This Smooth Muscle Actin antibody is un-conjugated
-
应用范围
- Western Blotting (WB), Immunofluorescence (IF), Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- An N-Terminal decapeptide of the alpha smooth muscle isoform was used as the immunogen for this Smooth muscle actin antibody.
- 克隆位点
- 1A4
- 亚型
- IgG2a kappa
- Top Product
- Discover our top product ACTA2 Primary Antibody
-
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibody
ACTA2 适用: 人, 大鼠, 小鼠, Cow WB, IHC, IF, ELISA, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyVerified ACTA2 适用: 人, 大鼠, 小鼠 WB, ELISA 宿主: 山羊 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyKO Validated ACTA2 适用: 人 WB, IF, IHC (p), ICC, IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (N-Term) antibodyACTA2 适用: 人, 大鼠 IHC, IF, FACS, StM 宿主: 小鼠 Monoclonal 1A4-ACTA2-791 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-124) antibodyACTA2 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 2-124) antibodyACTA2 适用: 人, 大鼠, 小鼠 WB, IHC, FACS, ELISA 宿主: 小鼠 Monoclonal 5C1H11 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 猴 WB, IHC, FACS, ELISA, ICC 宿主: 小鼠 Monoclonal 4A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 167-377) antibodyACTA2 适用: 小鼠 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) antibodyACTA2 适用: 人, 大鼠, 小鼠, 小鸡, 兔, 山羊 WB, IHC, ELISA, IHC (p), ICC, IHC (fro), IEM 宿主: 小鼠 Monoclonal alpha-SM1-1A4 unconjugated
anti-Actin, alpha 2, Smooth Muscle, Aorta (ACTA2) (AA 1-377) antibodyACTA2 适用: 人 WB, IF, ELISA 宿主: 小鼠 Monoclonal 4A8-2H3 unconjugated
-
- 应用备注
-
Variations in protocols, secondaries and substrates may require the Smooth muscle actin antibody to be titered up or down for optimal performance.
1. No special pretreatment is required for the immunohistochemical staining of FFPE tissue.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Western blot: 0.5-1 μg/mL,FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC(FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2) - 限制
- 仅限研究用
-
- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store the Smooth muscle actin antibody at 2-8°C (aliquot/freeze the azide-free format).
-
- 抗原
- Smooth Muscle Actin (ACTA2) (Actin, alpha 2, Smooth Muscle, Aorta (ACTA2))
- 别名
- Smooth Muscle Actin (ACTA2 产品)
- 别名
- AAT6 antibody, ACTSA antibody, MYMY5 antibody, GAMMA-ACTIN antibody, ACT antibody, ACTA3 antibody, ACTE antibody, ACTL3 antibody, ACTSG antibody, Act-4 antibody, Act4 antibody, SMGA antibody, 0610041G09Rik antibody, Actvs antibody, SMalphaA antibody, a-SMA antibody, alphaSMA antibody, wu:fb63d03 antibody, ASMA antibody, SMactin antibody, a-sma antibody, aat6 antibody, actsa antibody, mymy5 antibody, sm-actin antibody, ACTA2 antibody, actc1 antibody, ACT-4 antibody, asma antibody, ACTGE antibody, actin, alpha 2, smooth muscle, aorta antibody, actin, gamma 2, smooth muscle, enteric antibody, actin, alpha 2, smooth muscle, aorta L homeolog antibody, ACTA2 antibody, ACTG2 antibody, Actg2 antibody, Acta2 antibody, acta2 antibody, acta2.L antibody
- 背景
- Actin is a major component of the cytoskeleton and is present in most cell types. This Smooth muscle actin antibody is highly specific to the smooth muscle form. Its epitope lies in the first four N-terminal amino acids. The Smooth muscle actin antibody does not stain cardiac or skeletal muscle, however, it does stain myofibroblasts and myoepithelial cells. It could be used together with antimuscle specific actin and myogenin in making a diagnosis of smooth/skeletal muscle tumors. In most cases of rhabdomyosarcoma, this smooth form antibody yields negative results whereas antimuscle specific actin and myogenin are positive. Leiomyosarcomas are positive only with antimuscle specific actin and are negative with anti-myogenin.
- 基因ID
- 59
- 途径
- Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development
-
