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CD79a 抗体 (AA 202-216)

CD79A 适用: 人 FACS, IF, IHC (p) 宿主: 小鼠 Monoclonal JCB117 unconjugated
产品编号 ABIN3024107
发货至: 中国
  • 抗原 See all CD79a (CD79A) 抗体
    CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
    抗原表位
    • 69
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    AA 202-216
    适用
    • 184
    • 97
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    • 16
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    宿主
    • 101
    • 97
    • 1
    小鼠
    克隆类型
    • 116
    • 82
    • 1
    单克隆
    标记
    • 86
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    This CD79a antibody is un-conjugated
    应用范围
    • 113
    • 94
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    • 22
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    Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    纯化方法
    Protein G affinity chromatography
    免疫原
    Amino acids 202-216 (GTYQDVGSLNIADVQ) were used as the immunogen for this CD79a antibody.
    克隆位点
    JCB117
    亚型
    IgG1 kappa
    Top Product
    Discover our top product CD79A Primary Antibody
  • 应用备注
    The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the CD79a antibody to be titered up or down for optimal performance.

    1. Staining of formalin-fixed tissues requires boiling tissue sections in 10  mM citrate buffer,  pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.
    2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. FACS: 0.5-1 μg/million cells,IF: 0.5-1 μg/mL,IHC (FFPE): 0.5-1 μg/mL for 30 minutes at RT (1),Prediluted format : incubate for 30 min at RT (2)

    限制
    仅限研究用
  • 浓度
    0.2 mg/mL
    缓冲液
    0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Store the CD79a antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
  • 抗原
    CD79a (CD79A) (B-cell antigen receptor complex-associated protein alpha chain (CD79A))
    别名
    CD79a (CD79A 产品)
    别名
    CD79A antibody, Ig-alpha antibody, Iga antibody, Igalpha antibody, Ly-54 antibody, Ly54 antibody, mb-1 antibody, IGA antibody, MB-1 antibody, IG-alpha antibody, CD79a molecule antibody, CD79A antigen (immunoglobulin-associated alpha) antibody, Cd79a molecule-like antibody, CD79A antibody, Cd79a antibody, Cd79al antibody
    背景
    A disulphide-linked heterodimer, consisting of mb-1 (or CD79a) and B29 (or CD79b) polypeptides, is non-covalently associated with membrane-bound immunoglobulins on B cells. This complex of mb-1 and B29 polypeptides and immunoglobulin constitute the B-cell Ag receptor. CD79a first appears at pre B-cell stage, early in maturation, and persists until the plasma cell stage where it is found as an intracellular component. It is found in the majority of acute leukemias of B-cell precursors, lines and lymphomas, and in some myelomas. It is not present in myeloid or Tcell lines. CD79a antibody is generally used to complement CD20 mAb, especially for mature B-cell lymphomas after treatment with Rituximab (anti-CD20). This CD79a antibody will stain many of the same lymphomas as CD20 mAb, but also is more likely to stain B-lymphoblastic lymphoma/leukemia than is CD20 mAb. CD79a antibody also stains more cases of plasma cell myeloma and occasionally some types of endothelial cells as well.
    基因ID
    973
    途径
    BCR Signaling
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