Nuclear Antigen 抗体
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- 抗原
- Nuclear Antigen
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适用
- 人, 大鼠, 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Human cell nuclei were used as the immunogen for the Nuclear Antigen antibody.
- 克隆位点
- NUCM1-1
- 亚型
- IgG1 kappa
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anti-Nuclear Antigen antibody
适用: 人, 大鼠, 小鼠 IF, ICC, FACS, IHC 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 大鼠, 小鼠 IF, ICC, IHC (p) 宿主: 小鼠 Monoclonal NUCM1-1 unconjugated
anti-Nuclear Antigen antibody适用: 人 IF, ICC, FACS, IHC (p) 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, Non-Human Primate IF, ICC, FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
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- 应用备注
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Titering of the Nuclear Antigen antibody may be required for optimal performance.
1. FFPE testing requires sections to be boiled in pH 6 10 mM citrate buffer for 10-20 minutes, followed by cooling at RT for 20 minutes, prior to staining.
2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.\. Immunofluorescence: 0.5-1 μg/mL,Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,Immunohistochemistry (FFPE): 0.25-0.5 μg/mL for 30 min at RT (1),Prediluted format: incubate for 30 min at RT (2) - 限制
- 仅限研究用
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- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
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- 抗原
- Nuclear Antigen
- 背景
- This mAb recognizes an antigen associated with the nuclei in cells. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions.
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