Nuclear Antigen 抗体
-
- 抗原
- Nuclear Antigen
-
适用
- 人, 灵长类
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
-
应用范围
- Immunofluorescence (IF), Immunocytochemistry (ICC), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 无交叉反应
- 小鼠, 大鼠, 小鸡
- 产品特性
- This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Nuclei of human myeloid leukemia biopsy cells were used as the immunogen for this Nuclear antigen antibody.
- 克隆位点
- 235-1
- 亚型
- IgG1 kappa
-
anti-Nuclear Antigen antibody
适用: 人, 大鼠, 小鼠 IF, ICC, FACS, IHC 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, 大鼠, 小鼠 IF, ICC, IHC (p) 宿主: 小鼠 Monoclonal NUCM1-1 unconjugated
anti-Nuclear Antigen antibody适用: 人 IF, ICC, FACS, IHC (p) 宿主: 小鼠 Monoclonal NM106 unconjugated
anti-Nuclear Antigen antibody适用: 人, Non-Human Primate IF, ICC, FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal 235-1 unconjugated
anti-Nuclear Antigen antibody适用: 人, 灵长类 IF, ICC, FACS, IHC (fro) 宿主: 小鼠 Monoclonal 235-1 unconjugated
-
- 应用备注
-
The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the Nuclear antigen antibody to be titered up or down for optimal performance.
1. Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Citrate Buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes.\. IHC (Frozen): 0.5-1 μg/mL for 30 minutes at RT (1),Immunocytochemistry (Acetone-fixed): 0.25-0.5 μg/mL for 30 min at RT,FACS: 0.5-1 μg/10e6 cells,IF: 0.5-1 μg/mL
- 限制
- 仅限研究用
-
- 浓度
- 0.2 mg/mL
- 缓冲液
- 0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store the Nuclear Antigen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
-
- 抗原
- Nuclear Antigen
- 背景
- This nuclear antigen antibody is part of a panel of reagents which recognizes subcellular organelles or compartments. These markers may be useful in identification of these organelles in cells, tissues, and biochemical preparations. Clone 235-1 antibody specifically detects an antigen associated with the human nuclei. It can be used to stain the nuclei in cell or tissue preparations and can be used as a nuclear marker in subcellular fractions. It produces a speckled pattern in normal and malignant cells and may be used to stain the nuclei of cells in fixed or frozen tissue sections. The nuclear antigen antibody can also be used with paraformaldehyde fixed frozen tissue or cell preparations.
-
