ATG5 抗体 (Internal Region)
Quick Overview for ATG5 抗体 (Internal Region) (ABIN302179)
抗原
See all ATG5 抗体适用
宿主
克隆类型
标记
应用范围
质量等级
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抗原表位
- Internal Region
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原理
- ATG5
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序列
- SQVINEMQKK DHKQ
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交叉反应
- Cow, 人, 小鼠
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纯化方法
- Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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免疫原
- Peptide with sequence C-SQVINEMQKKDHKQ, from the internal region of the protein sequence according to NP_004840.1.
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亚型
- IgG
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应用备注
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Western Blot: Preliminary testing showed a band at approx. 28-30 kDa in lysate of cell line MDA-MB-231, after 1 μg/mL antibody staining (calculated Mwt. Of 32.4 kDa according to NP_004840.1). Primary incubation 1 hour at room temperature.
Peptide ELISA: antibody detection limit dilution 1:64000.
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限制
- 仅限研究用
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状态
- Liquid
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浓度
- 0.5 mg/mL
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缓冲液
- Supplied at 0.5 mg/mL in Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin.
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储存液
- Sodium azide
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注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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注意事项
- Minimize freezing and thawing.
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储存条件
- -20 °C
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储存方法
- Aliquot and store at -20°C, with minimal freeze/thawing. A working aliquot may be refrigerated at 4°C for a few weeks and still remain viable.
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- ATG5 (ATG5 Autophagy Related 5 (ATG5))
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别名
- ATG5
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背景
- ATG5, ATG5 autophagy related 5 homolog (S. cerevisiae), APG5, APG5L, ASP, hAPG5, APG5 (autophagy 5, S. cerevisiae)-like, APG5 autophagy 5-like, ATG5 autophagy related 5-like, OTTHUMP00000040507, apoptosis specific protein
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基因ID
- 9474, 11793
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NCBI登录号
- NP_004840
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途径
- Activation of Innate immune Response, Production of Molecular Mediator of Immune Response, Autophagy
抗原
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