His Tag 抗体
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- 抗原 See all His Tag 抗体
- His Tag
- 适用
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This His Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA
- 序列
- HHHHHH
- 特异性
- The 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells
- 纯化方法
- Affinity purified
- 免疫原
- 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
- Top Product
- Discover our top product His Tag Primary Antibody
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anti-His Tag antibody
WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibodyWB, ELISA, IP, IHC, FACS 宿主: 小鼠 Monoclonal 33D10-D2-G8 unconjugated
anti-His Tag antibody适用: 人 WB 宿主: 小鼠 Monoclonal 6AT18 unconjugated
anti-His Tag antibodyWB, ELISA, IP, IF, FACS 宿主: 小鼠 Monoclonal 6G2A9 unconjugated
anti-His Tag antibody适用: 标记, 人 WB 宿主: 小鼠 Monoclonal 2D5 unconjugated
anti-His Tag antibody (HRP)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 HRP
anti-His Tag (C-Term) antibodyWB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-His Tag antibody (Biotin)WB, ELISA, IP 宿主: 小鼠 Monoclonal 33D10-D2 Biotin
anti-His Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated
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- 应用备注
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Approved: ELISA (1:10000 - 1:40000), WB (1:1000 - 1:3000)
Usage: This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. - 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Aliquot to Avoid freeze/thaw cycles. Store undiluted.
- 储存条件
- -20 °C
- 储存方法
- Store at -20°C. Aliquot to avoid freeze-thaw cycles. Store undiluted.
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- 抗原
- His Tag
- Abstract
- His Tag 产品
- 物质类
- Tag
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