WNT2B 抗体
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- 抗原 See all WNT2B 抗体
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
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适用
- 人, 小鼠
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宿主
- 山羊
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克隆类型
- 多克隆
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标记
- This WNT2B antibody is un-conjugated
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应用范围
- Western Blotting (WB), ELISA, Immunocytochemistry (ICC)
- 特异性
- Selected for its ability to recognize mouse and human Wnt-2b in the applications listed.
- 纯化方法
- Affinity purified
- 免疫原
- E. coli-derived rmWnt-2b.
- 亚型
- IgG
- Top Product
- Discover our top product WNT2B Primary Antibody
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anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 301-391) antibody
WNT2B 适用: 小鼠, 大鼠 WB, ELISA, IHC (p), IF (cc), IF (p), IHC (fro) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 87-112), (N-Term) antibodyWNT2B 适用: 人, 小鼠, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (Middle Region) antibodyWNT2B 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马 WB, IHC 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 62-391) antibodyWNT2B 适用: 人 WB, IHC, ICC, IP 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term) antibodyWNT2B 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 马, Pig, 猴, Bat, Hamster IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (AA 229-278) antibodyWNT2B 适用: 人, 小鼠, 大鼠, Cow, 兔, 犬, 豚鼠, 马, Pig, 猴, Bat WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term) antibodyWNT2B 适用: 人, 豚鼠, Pig WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) (N-Term) antibodyWNT2B 适用: 人, Cow, Pig, 猴 WB, IHC, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B) antibodyWNT2B 适用: 人 IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Approved: ELISA, ICC, WB
Usage: Western Blot: This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Wnt-2b. The detection limit for rmWnt-2b is approximately 5 ng/lane and 1 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows approximately 20 % cross-reactivity with rhWnt-2, and less than 5 % cross-reactivity with rhWnt-7a, -9a, rmWnt-1, -3a, -4, -5a, -5b, -8a, -8b, -9b, -10a, -10b, -11, -16. Immunocytochemistry: This antibody has been used at a concentration of 10 μg/mL to detect Wnt-2b in BT20 human breast carcinoma cells. Cells were fixed with PBS containing 4 % paraformaldehyde for 20 minutes at room temperature and blocked with PBS containing 10 % normal donkey serum, 0.1 % Triton X-100, and 1 % BSA for 45 minutes at room temperature. After blocking, cells were incubated with diluted primary antibody overnight at 4? C followed by NorthernLights-557-coupled anti-goat IgG at room temperature in the dark for one hour. Between each step, cells were washed with PBS containing BSA. Direct ELISA: This antibody can be used at 0.5-1.0 μg/mL with the appropriate secondary reagents to detect mouse Wnt-2b. The detection limit for rmWnt-2b is approximately 0.5 ng/well. - 说明
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Target Species of Antibody: Mouse
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- deionized water
- 浓度
- Lot specific
- 缓冲液
- Lyophilized.
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- 抗原
- WNT2B (Wingless-Type MMTV Integration Site Family, Member 2B (WNT2B))
- 别名
- WNT2B (WNT2B 产品)
- 别名
- WNT13 antibody, XWNT2 antibody, wnt2b antibody, XWnt-2 antibody, Xwnt-2b antibody, wnt-2b antibody, xwnt2b antibody, WNT2B antibody, Wnt13 antibody, Wnt family member 2B antibody, wingless-type MMTV integration site family, member 2Ba antibody, Wnt family member 2B L homeolog antibody, wingless-type MMTV integration site family, member 2B antibody, wingless-type MMTV integration site family, member 2Bb antibody, WNT2B antibody, Wnt2b antibody, wnt2ba antibody, wnt2b.L antibody, wnt2bb antibody
- 背景
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Name/Gene ID: WNT2B
Family: WNT
Synonyms: WNT2B, Protein Wnt-2b, XWNT2, Protein Wnt-13, WNT13, XWNT2, Xenopus, homolog of - 基因ID
- 7482
- UniProt
- Q93097
- 途径
- WNT signaling
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