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p53 抗体

Name: p53 抗体
Brand: antibodies-online
SKU: ABIN2855525
Availability: OutOfStock

This anti-p53 antibody is a 兔多克隆 antibody detecting p53 in WB, IF, IHC (p), IP, ICC 和 ChIP. Suitable for 人. This Primary Antibody has been cited in 1 publication.

WB Image Sample (30 ug of whole cell lysate) A: A431 , 10% SDS PAGE antibody diluted at 1:1000 (p53 抗体)

ICC/IF Image Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed U2OS, using p53, antibody (Green) at 1:500 dilution. (p53 抗体)

IHC-P Image Immunohistochemical analysis of paraffin-embedded Cal27 xenograft, using p53, antibody at 1:500 dilution. (p53 抗体)

IHC

ChIP

IP Image Immunoprecipitation of p53 protein. (p53 抗体)

ICC/IF Image p53 antibody detects p53 protein at nucleus by immunofluorescent analysis. (p53 抗体)

HDAC2 positively regulates the expression of Sp1 and negatively regulates the expression of p53 in ER+ breast cancer cells. (p53 抗体)

8 images

(1 reference)

产品编号 ABIN2855525

发货至: 中国

Quick Overview for p53 抗体 (ABIN2855525)

抗原

See all p53 (TP53) 抗体

p53 (TP53) (Tumor Protein P53 (TP53))

适用

人

宿主

兔

克隆类型

多克隆

标记

This p53 antibody is un-conjugated

应用范围

Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Immunocytochemistry (ICC), Chromatin Immunoprecipitation (ChIP)

质量等级

KO Validated

Discover our top product TP53 Primary Antibody

交叉反应

人, 小鼠, 斑马鱼

产品特性

Rabbit Polyclonal antibody to p53 (tumor protein p53)
p53 antibody

纯化方法

Purified by antigen-affinity chromatography.

免疫原

Recombinant protein encompassing a sequence within the center region of human p53. The exact sequence is proprietary.

亚型

IgG
anti-Tumor Protein P53 (TP53) (pSer392) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 1-100) antibody
TP53 适用: 人 WB, IHC, IP 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (N-Term) antibody
TP53 适用: 人 WB, IF, IHC (p), IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 301-393) antibody
TP53 适用: 人, 小鼠, 大鼠, 山羊 WB, ELISA, FACS, IHC (p), ICC, IF (p), IF (cc), IHC (fro) 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (pSer33) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IF, IHC (p), IP 宿主: 小鼠 Monoclonal 2C3 unconjugated

anti-Tumor Protein P53 (TP53) (AA 94-201) antibody
TP53 适用: 人 WB, ELISA, IHC (p), PLA, RNAi 宿主: 小鼠 Monoclonal 2C11 unconjugated

anti-Tumor Protein P53 (TP53) (pSer20) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (pSer15) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Tumor Protein P53 (TP53) (acLys319) antibody
TP53 适用: 人, 小鼠, 大鼠 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IP: 1:100-1:500. Optimal dilutions/concentrations should be determined by the researcher. Not tested in other applications.

说明

Positive Control: HCT116 cells with 30uM cisplatin treatment for 24hr
Validation: KO/KD, Orthogonal

限制

仅限研究用
状态

Liquid

浓度

1.28 mg/mL

缓冲液

1XPBS pH 7, 20 % Glycerol, 0.025 % ProClin 300

储存液

ProClin

注意事项

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

储存条件

4 °C,-20 °C

储存方法

Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4°C. For long-term storage, aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
Chen, Lin, Liang, Villalobos, Villaflores, Lou, Lai, Hsiao: "UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish." in: Animals : an open access journal from MDPI, Vol. 10, Issue 6, (2020) (PubMed).
抗原

p53 (TP53) (Tumor Protein P53 (TP53))

别名

tumor protein p53

背景

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Cellular Localization: Cytoplasm , Nucleus , Endoplasmic reticulum

分子量

44 kDa

基因ID

7157

UniProt

P04637

途径

p53 Pathway, MAPK Pathway, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect