电话:
+86 (0512) 65829739
传真:
+86 (010) 6788 5057
电子邮件:
orders@antibodies-online.cn

RAD51 抗体 (Center)

This anti-RAD51 antibody is a 兔 多克隆 antibody detecting RAD51 in WB, IP, IHC (p), IF 和 ICC. Suitable for 人 和 小鼠. This Primary Antibody has been cited in 4+ publications.
产品编号 ABIN2854762
发货至: 中国

Quick Overview for RAD51 抗体 (Center) (ABIN2854762)

抗原

See all RAD51 抗体
RAD51 (DNA Repair Protein Homolog 1 (RAD51))

适用

  • 165
  • 75
  • 38
  • 4
  • 4
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
人, 小鼠

宿主

  • 106
  • 62
  • 1

克隆类型

  • 97
  • 72
多克隆

标记

  • 82
  • 9
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 4
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 3
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
This RAD51 antibody is un-conjugated

应用范围

  • 116
  • 41
  • 40
  • 38
  • 29
  • 29
  • 21
  • 20
  • 10
  • 10
  • 10
  • 9
  • 4
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF), Immunocytochemistry (ICC)
  • 抗原表位

    • 48
    • 23
    • 15
    • 11
    • 7
    • 6
    • 5
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center

    交叉反应

    小鸡, 犬, 兔, 大鼠, 斑马鱼, 牛, Xenopus tropicalis

    交叉反应 (详细)

    Chicken (95 %), Dog (98 %), Rabbit (97 %), Rat (98 %), Zebrafish (90 %), Bovine (98 %), Xenopus tropicalis (97 %)

    产品特性

    Rabbit Polyclonal antibody to Rad51 (RAD51 homolog (RecA homolog, E. coli) (S. cerevisiae))
    Rad51 antibody [N1C2]

    纯化方法

    Purified by antigen-affinity chromatography.

    免疫原

    Recombinant protein encompassing a sequence within the center region of human Rad51. The exact sequence is proprietary.

    亚型

    IgG
  • 应用备注

    Suggested dilution Reference ICC/IF 1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections) 1:100-1:1000* Immunoprecipitation 1:100-1:500* Western blot 1:500-1:3000* Not tested in other applications. *Optimal dilutions/concentrations should be determined by the researcher.Suggested dilutionReferenceICC/IF1:100-1:1000* IHC (Formalin-fixed paraffin-embedded sections)1:100-1:1000* Immunoprecipitation1:100-1:500* Western blot1:500-1:3000*

    说明

    Positive Control: 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , NIH-3T3

    限制

    仅限研究用
  • 状态

    Liquid

    浓度

    0.57 mg/mL

    缓冲液

    0.1M Tris, 0.1M Glycine, 10 % Glycerol ( pH 7). 0.01 % Thimerosal was added as a preservative.

    储存液

    Thimerosal (Merthiolate)

    注意事项

    This product contains Thimerosal (Merthiolate): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    储存条件

    -20 °C

    储存方法

    Keep as concentrated solution. Aliquot and store at -20°C or below. Avoid multiple freeze-thaw cycles.
  • Chen, Lin, Liang, Villalobos, Villaflores, Lou, Lai, Hsiao: "UVB Irradiation Induced Cell Damage and Early Onset of Junbb Expression in Zebrafish." in: Animals : an open access journal from MDPI, Vol. 10, Issue 6, (2020) (PubMed).

    Nacson, Krais, Bernhardy, Clausen, Feng, Wang, Nicolas, Cai, Tricarico, Hua, DiMarcantonio, Martinez, Zong, Handorf, Bellacosa, Testa, Nussenzweig, Gupta, Sykes, Johnson: "BRCA1 Mutation-Specific Responses to 53BP1 Loss-Induced Homologous Recombination and PARP Inhibitor Resistance." in: Cell reports, Vol. 24, Issue 13, pp. 3513-3527.e7, (2019) (PubMed).

    Chakraborty, Tapryal, Venkova, Horikoshi, Pandita, Sarker, Sarkar, Pandita, Hazra: "Classical non-homologous end-joining pathway utilizes nascent RNA for error-free double-strand break repair of transcribed genes." in: Nature communications, Vol. 7, pp. 13049, (2018) (PubMed).

    Ge, Che, Du: "The UBC Domain Is Required for BRUCE to Promote BRIT1/MCPH1 Function in DSB Signaling and Repair Post Formation of BRUCE-USP8-BRIT1 Complex." in: PLoS ONE, Vol. 10, Issue 12, pp. e0144957, (2015) (PubMed).

  • 抗原

    RAD51 (DNA Repair Protein Homolog 1 (RAD51))

    别名

    Rad51

    背景

    The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist.

    分子量

    37 kDa

    基因ID

    5888

    途径

    DNA Damage Repair
You are here:
Chat with us!