NEDD8 抗体
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- 抗原 See all NEDD8 抗体
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
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适用
- 人, 小鼠, 大鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This NEDD8 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
- This antibody detects endogenous levels of NEDD8 protein. (region surrounding Asp21)
- 交叉反应 （详细）
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - 纯化方法
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen
- 纯度
- > 95 % pure by SDS-PAGE
- Top Product
- Discover our top product NEDD8 Primary Antibody
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anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibody
NEDD8 适用: 人 WB, IHC, ELISA, FACS, ICC 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, ELISA, IF, IHC (p), FACS 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴, Cow, 非洲爪蟾 WB, IHC, IF, IHC (p), FACS, IP 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: 人 WB, IF, IHC (p), FACS, EIA 宿主: 小鼠 Monoclonal 1A7 unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-81) antibodyNEDD8 适用: 人 WB, IHC, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) antibodyNEDD8 适用: Saccharomyces cerevisiae WB 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (AA 1-32) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8) (N-Term) antibodyNEDD8 适用: 人 WB, IHC, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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ELISA: 1: 5000approx. 1: 10000. WB: 1: 500approx. 1: 1000. IHC: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1,0 mg/mL
- 缓冲液
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- DO NOT FREEZE!
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C.
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- 抗原
- NEDD8 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 8 (NEDD8))
- 别名
- NEDD8 (NEDD8 产品)
- 别名
- NEDD-8 antibody, Rub1 antibody, CG10679 antibody, Dmel\\CG10679 antibody, NEDD8 antibody, dNEDD8 antibody, nedd8 antibody, ubiquitin antibody, si:zc14a17.5 antibody, GB17785 antibody, DDBDRAFT_0218116 antibody, DDBDRAFT_0238041 antibody, DDB_0218116 antibody, DDB_0238041 antibody, nedd8.S antibody, neural precursor cell expressed, developmentally down-regulated 8 antibody, neural precursor cell expressed, developmentally down-regulated gene 8 antibody, CG10679 gene product from transcript CG10679-RB antibody, Nedd8 antibody, ubiquitin-like protein Nedd8 antibody, neural precursor cell expressed, developmentally down-regulated 8 L homeolog antibody, NEDD8 antibody, Nedd8 antibody, nedd8 antibody, nedd8.L antibody
- 背景
- NEDD8 is a ubiquitin (Ub)-like molecule that shares 80 % homology with ubiquitin, a protein known to modify and target various proteins for proteolytic degradation. NEDD8 and the corresponding yeast homolog RUB1 are activated by the E1 ubiquitin activating enzyme UBA2, which forms isopeptide linkages between thio esters. Similar to the Ub-mediated proteolytic pathway, NEDD8 is covalently coupled to an E3 Ub ligase by the E2 conjugating enzyme, UBC12. NEDD8 preferentially associates with the CUL-2 protein in the E3 ligase complex CBCVHL, which consists of cullin proteins associating with elongin B/C and the tumor suppressor F-box protein von Hippel Lindau (VHL). NEDD8 is predominantly localized to the nucleus and is highly expressed in adult heart and skeletal muscle. In vitro studies indicate that NEDD8 tetramers are also able to bind to the 26S proteasome, and that they assemble into complexes with conserved Ub-like moieties, suggesting that NEDD8 may regulate proteolysis of intracellular proteins similar to other Ub-mediated pathways.Synonyms: Neural precursor cell expressed developmentally down-regulated protein 8
- 分子量
- approx. 85 kDa
- 基因ID
- 4738
- NCBI登录号
- NP_006147
- UniProt
- Q15843
- 途径
- Ubiquitin Proteasome Pathway
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