(1 reference)-
- 抗原 See all IL-10RA (IL10RA) 抗体
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
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适用
- 小鼠
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宿主
- 大鼠
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克隆类型
- 单克隆
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标记
- This IL-10RA antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS)
- 品牌
- BD Pharmingen™
- 产品特性
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The 1B1.3a antibody reacts with the extracellular region of mouse CD210 which is also known as the mouse IL-10 receptor (mIL-10R), it does not recognize the human IL-10R. The IL-10R is expressed by a variety of mouse cell types and cell lines including thymocytes, T cells, B cells, and monocytes. 1B1.3a is a neutralizing antibody and reportedly blocks the binding of human IL-10, which cross-reacts with the mouse IL-10R. The immunogen used for the generation of the 1B1.3a hybridoma was purified recombinant ligand-binding domain of mIL-10R. Mouse B220+ splenocytes were stained with purified 1B1.3a (0.25 μg, Cat. No. 559912) followed by biotinylated polyclonal goat anti-rat Ig (0.25 μg, Cat. No. 554014) and Streptavidin-PE (0.015 μg, Cat. No. 554061) in a three layer staining protocol to amplify immunofluorescent signals (left panel). In addition, cells in buffer containing sodium azide were pre-incubated on ice with recombinant mouse IL-10 (0.25 μg, Cat. No. 554585) prior to staining with 1B1.3a to determine the blocking effect of bound IL-10 protein on staining with 1B1.3a (right panel). Staining with the purified 1B1.3a antibody (filled histograms) is compared to the staining obtained with the secondary antibody alone (open histograms). Histograms in both panels are gated on the lymphocyte population as defined by light scattering characteristics and were also gated on the cell subset stained with FITC-conjugated rat-anti-mouse B220 (0.06 μg, Cat. No. 553088).
BD Pharmingen™ Purified Rat Anti-Mouse CD210 - Purified - Clone 1B1.3a - Isotype Rat IgG1, κ - Reactivity Ms - 0.5 mg - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Purified recombinant ligand-binding domain of mIL-10R
- 克隆位点
- 1B1-3a
- 亚型
- IgG1 kappa
- Top Product
- Discover our top product IL10RA Primary Antibody
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anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 132-235) antibody
IL10RA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 22-230) antibodyIL10RA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) antibodyIL10RA 适用: 人, 小鼠, 大鼠 IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (C-Term) antibodyIL10RA 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (pTyr496) antibodyIL10RA 适用: 人 WB, ELISA, IF, ICC 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (Ser560) antibodyIL10RA 适用: 人 WB, ELISA, IF 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 128-177) antibodyIL10RA 适用: 人, Cow, Pig, 马, 猴 WB 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) antibodyIL10RA 适用: 人 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 147-175) antibodyIL10RA 适用: 人 WB, FACS, IF 宿主: 兔 Polyclonal RB23343 unconjugated
anti-Interleukin 10 Receptor, alpha (IL10RA) (AA 81-180) antibodyIL10RA 适用: 人 WB, ELISA 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 限制
- 仅限研究用
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- 浓度
- 0.5 mg/mL
- 缓冲液
- Aqueous buffered solution containing ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C.
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: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).
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: "IL-10 inhibits macrophage activation and proliferation by distinct signaling mechanisms: evidence for Stat3-dependent and -independent pathways." in: The EMBO journal, Vol. 17, Issue 4, pp. 1006-18, (1998) (PubMed).
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- 抗原
- IL-10RA (IL10RA) (Interleukin 10 Receptor, alpha (IL10RA))
- 别名
- CD210 (IL10RA 产品)
- 别名
- IL10R1 antibody, IL10RA antibody, CD210 antibody, CD210a antibody, CDW210A antibody, HIL-10R antibody, IL-10R1 antibody, IL10R antibody, AW553859 antibody, CDw210 antibody, CDw210a antibody, Il10r antibody, mIL-10R antibody, interleukin 10 receptor subunit alpha antibody, interleukin 10 receptor, alpha antibody, IL10RA antibody, Il10ra antibody
- 背景
- Synonyms: IL-10 Receptor
- 途径
- Growth Factor Binding
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